Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Non-thermal plasma application in tumor-bearing mice induces increase of serum hmgb1. / Troitskaya, Olga; Golubitskaya, Ekaterina; Biryukov, Mikhail и др.
в: International Journal of Molecular Sciences, Том 21, № 14, 5128, 20.07.2020, стр. 1-14.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Non-thermal plasma application in tumor-bearing mice induces increase of serum hmgb1
AU - Troitskaya, Olga
AU - Golubitskaya, Ekaterina
AU - Biryukov, Mikhail
AU - Varlamov, Mikhail
AU - Gugin, Pavel
AU - Milakhina, Elena
AU - Richter, Vladimir
AU - Schweigert, Irina
AU - Zakrevsky, Dmitry
AU - Koval, Olga
N1 - Funding Information: Funding: The authors gratefully acknowledge financial support from Russian Science Foundation grant # 19–19–00255, Russian State funded budget project of ICBFM SB RAS # АААА-А17–117020210023–1, RFBR grant # 19–34–90134 for young scientists. The authors, E.M. and D.Z., were also partly supported by RFBR # 18–08– 00510. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/7/20
Y1 - 2020/7/20
N2 - The application of cold atmospheric plasma (CAP) in cancer therapy could be one of the new anticancer strategies. In the current work, we used cold atmospheric plasma jet for the treatment of cultured cells and mice. We showed that CAP induced the death of MX−7 mouse rhabdomyosarcoma cells with the hallmarks of immunogenic cell death (ICD): calreticulin and heat shock protein 70 (HSP70) externalization and high-mobility group box 1 protein (HMGB1) release. The intensity of HMGB1 release after the CAP treatment correlated directly with the basal extracellular HMGB1 level. Releasing from dying cells, HMGB1 can act as a proinflammatory cytokine. Our in vivo study demonstrated that cold atmospheric plasma induces a short-term two-times increase in serum HMGB1 level only in tumor-bearing mice with no effect in healthy mice. These findings support our hypothesis that CAP-dependent HMGB1 release from dying cancer cells can change the serum HMGB1 level. At the same time, we showed a weak cytokine response to CAP irradiation in healthy mice that can characterize CAP as an immune-safety physical antitumor approach.
AB - The application of cold atmospheric plasma (CAP) in cancer therapy could be one of the new anticancer strategies. In the current work, we used cold atmospheric plasma jet for the treatment of cultured cells and mice. We showed that CAP induced the death of MX−7 mouse rhabdomyosarcoma cells with the hallmarks of immunogenic cell death (ICD): calreticulin and heat shock protein 70 (HSP70) externalization and high-mobility group box 1 protein (HMGB1) release. The intensity of HMGB1 release after the CAP treatment correlated directly with the basal extracellular HMGB1 level. Releasing from dying cells, HMGB1 can act as a proinflammatory cytokine. Our in vivo study demonstrated that cold atmospheric plasma induces a short-term two-times increase in serum HMGB1 level only in tumor-bearing mice with no effect in healthy mice. These findings support our hypothesis that CAP-dependent HMGB1 release from dying cancer cells can change the serum HMGB1 level. At the same time, we showed a weak cytokine response to CAP irradiation in healthy mice that can characterize CAP as an immune-safety physical antitumor approach.
KW - Anticancer treatment
KW - HMGB1
KW - Immunogenic cell death
KW - Non-thermal plasma
KW - Proinflammatory cytokines
UR - http://www.scopus.com/inward/record.url?scp=85088162308&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=45410715
U2 - 10.3390/ijms21145128
DO - 10.3390/ijms21145128
M3 - Article
C2 - 32698492
AN - SCOPUS:85088162308
VL - 21
SP - 1
EP - 14
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 14
M1 - 5128
ER -
ID: 24783261