TY - JOUR

T1 - New Opportunities for Preoperative Diagnosis of Medullary Thyroid Carcinoma

AU - Lukyanov, Sergei A

AU - Sergiyko, Sergei V

AU - Titov, Sergei E

AU - Beltsevich, Dmitry G

AU - Veryaskina, Yulia A

AU - Vanushko, Vladimir E

AU - Urusova, Liliya S

AU - Mikheenkov, Alexander A

AU - Kozorezova, Evgeniya S

AU - Vorobyov, Sergey L

AU - Sleptsov, Ilya V

N1 - Funding: The study was funded by the Ministry of Science and Higher Education (No. 121040100268-9) and by the Institute of Molecular and Cell Biology, Siberian Branch of the Russian Academy of Sciences (project No. FWGZ-2021-0014).

PY - 2023/5/18

Y1 - 2023/5/18

N2 - The preoperative diagnostics of medullary thyroid carcinoma (MTC), including the measuring of the blood calcitonin level, has a number of limitations. Particular focus has recently been placed on the role of miRNAs in the development of various malignant tumors; a comparative analysis of accuracy of the existing methods for MTC diagnosis with a novel diagnosis method, evaluation of the miRNA-375 expression level, was performed in this study. The expression level of miRNA-375 in cytology samples from 555 patients with the known histological diagnosis, including 41 patients with confirmed postoperative diagnosis of MTC, was assessed. The diagnostic parameters of the basal calcitonin level, calcitonin in wash-out fluid from the FNAB needle, and miRNA-375 were compared. An assessment of the miRNA-375 expression level made it possible to detect all the MTC samples with a 100% accuracy among all the 555 cytology specimens, as well as in non-informative FNAB specimens, and specimens from the ipsilateral thyroid lobe. Parameters such as sensitivity, specificity, PPV, and NPV were 100%. The miRNA-375 level, unlike calcitonin, does not correlate with tumor volume, so it does not have the so-called "gray zone". An assessment of the miRNA-375 expression allows one to accurately distinguish MTC from other malignant and benign thyroid tumors.

AB - The preoperative diagnostics of medullary thyroid carcinoma (MTC), including the measuring of the blood calcitonin level, has a number of limitations. Particular focus has recently been placed on the role of miRNAs in the development of various malignant tumors; a comparative analysis of accuracy of the existing methods for MTC diagnosis with a novel diagnosis method, evaluation of the miRNA-375 expression level, was performed in this study. The expression level of miRNA-375 in cytology samples from 555 patients with the known histological diagnosis, including 41 patients with confirmed postoperative diagnosis of MTC, was assessed. The diagnostic parameters of the basal calcitonin level, calcitonin in wash-out fluid from the FNAB needle, and miRNA-375 were compared. An assessment of the miRNA-375 expression level made it possible to detect all the MTC samples with a 100% accuracy among all the 555 cytology specimens, as well as in non-informative FNAB specimens, and specimens from the ipsilateral thyroid lobe. Parameters such as sensitivity, specificity, PPV, and NPV were 100%. The miRNA-375 level, unlike calcitonin, does not correlate with tumor volume, so it does not have the so-called "gray zone". An assessment of the miRNA-375 expression allows one to accurately distinguish MTC from other malignant and benign thyroid tumors.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85160765290&origin=inward&txGid=ec9157f805012c4b729375e5db143e25

U2 - 10.3390/biomedicines11051473

DO - 10.3390/biomedicines11051473

M3 - Article

C2 - 37239145

VL - 11

JO - Biomedicines

JF - Biomedicines

SN - 2227-9059

IS - 5

M1 - 1473

ER -