TY - JOUR

T1 - New model systems for RNA interference in plants to develop eco-friendly and effective antipathogenic tools

AU - Urin, A. V.

AU - Golubeva, T. S.

N1 - Publisher Copyright: © Published under licence by IOP Publishing Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/4/12

Y1 - 2021/4/12

N2 - The methods which are used to combat pathogens of crops, in particular, chemical treatment, pose a threat to environmental safety. RNA interference could be one of the best ways to selectively control pests, but there are not enough methods for introducing dsRNA into plants without creating GMOs. The development of new methods of dsRNA delivery requires affordable and fast gene model systems, the purpose of which is our work. We selected the genes of several carbonic anhydrases functioning in the thylakoids and mitochondria of the whole plant, the suppression of which will be seen phenotypically based on the data on mutants for these genes. Specific primers have been designed, and a PCR reaction has also been carried out to obtain the product of the corresponding mRNA, which will be delivered to the plant in the future using the VIGS system.

AB - The methods which are used to combat pathogens of crops, in particular, chemical treatment, pose a threat to environmental safety. RNA interference could be one of the best ways to selectively control pests, but there are not enough methods for introducing dsRNA into plants without creating GMOs. The development of new methods of dsRNA delivery requires affordable and fast gene model systems, the purpose of which is our work. We selected the genes of several carbonic anhydrases functioning in the thylakoids and mitochondria of the whole plant, the suppression of which will be seen phenotypically based on the data on mutants for these genes. Specific primers have been designed, and a PCR reaction has also been carried out to obtain the product of the corresponding mRNA, which will be delivered to the plant in the future using the VIGS system.

UR - http://www.scopus.com/inward/record.url?scp=85104894067&partnerID=8YFLogxK

U2 - 10.1088/1755-1315/723/2/022040

DO - 10.1088/1755-1315/723/2/022040

M3 - Conference article

AN - SCOPUS:85104894067

VL - 723

JO - IOP Conference Series: Earth and Environmental Science

JF - IOP Conference Series: Earth and Environmental Science

SN - 1755-1307

IS - 2

M1 - 022040

T2 - 2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021

Y2 - 25 January 2021

ER -