TY - JOUR

T1 - New data on comparative cytogenetics of the mouse-like hamsters (Calomyscus thomas, 1905) from iran and turkmenistan

AU - Romanenko, Svetlana A.

AU - Malikov, Vladimir G.

AU - Mahmoudi, Ahmad

AU - Golenishchev, Feodor N.

AU - Lemskaya, Natalya A.

AU - Pereira, Jorge C.

AU - Trifonov, Vladimir A.

AU - Serdyukova, Natalia A.

AU - Ferguson-Smith, Malcolm A.

AU - Aliabadian, Mansour

AU - Graphodatsky, Alexander S.

N1 - Funding Information: Funding: This research was funded by the Russian Science Foundation (RSF), grant number No. 19-14-00034 (A.S.G.). Significant funding was also provided by the RFBR grants № 19-04-00557a and Federal State Program ZIN RAS № AAAA-A19-119032590102-7 (F.N.G.) for field sampling. Funding Information: Acknowledgments: The authors gratefully acknowledge the resources provided by the “Molecular and Cellular Biology” core facility of the IMCB SB RAS (0310-2018-0011 grant). We thank Vladimir S. Lebedev from Zoological museum, moscow State University, moscow, Russia, for providing animals from the laboratory colony of moscow Zoo. We are also highly obliged to Touraj Sayyadpour, Kordiyeh Hamidi, and Safieh Akbarirad (researchers at the faculty of biology, Ferdowsi University of mashhad), morteza Radmanesh (Laboratory technician of the faculty of biology, Fer-dowsi University of mashhad), Ahmad Abedipour (driver of the Ferdowsi University of mashhad) for assistance in obtaining the materials. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/6

Y1 - 2021/6

N2 - The taxonomy of the genus Calomyscus remains controversial. According to the latest systematics the genus includes eight species with great karyotypic variation. Here, we studied karyotypes of 14 Calomyscus individuals from different regions of Iran and Turkmenistan using a new set of chromosome painting probes from a Calomyscus sp. male (2n = 46, XY; Shahr-e-Kord-Soreshjan-Cheshme maiak Province). We showed the retention of large syntenic blocks in karyotypes of individuals with identical chromosome numbers. The only rearrangement (fusion 2/21) differentiated Calomyscus elburzensis, Calomyscus mystax mystax, and Calomyscus sp. from Isfahan Province with 2n = 44 from karyotypes of C. bailwardi, Calomyscus sp. from Shahr-e-Kord, Chahar mahal and Bakhtiari-Aloni, and Khuzestan-Izeh Provinces with 2n = 46. The individuals from Shahdad tunnel, Kerman Province with 2n = 51–52 demonstrated non-centric fissions of chromosomes 4, 5, and 6 of the 46-chromosomal form with the formation of separate small acrocentrics. A heteromorphic pair of chromosomes in a specimen with 2n = 51 resulted from a fusion of two autosomes. C-banding and chromomycin A3-DAPI staining after G-banding showed extensive heterochromatin variation between individuals.

AB - The taxonomy of the genus Calomyscus remains controversial. According to the latest systematics the genus includes eight species with great karyotypic variation. Here, we studied karyotypes of 14 Calomyscus individuals from different regions of Iran and Turkmenistan using a new set of chromosome painting probes from a Calomyscus sp. male (2n = 46, XY; Shahr-e-Kord-Soreshjan-Cheshme maiak Province). We showed the retention of large syntenic blocks in karyotypes of individuals with identical chromosome numbers. The only rearrangement (fusion 2/21) differentiated Calomyscus elburzensis, Calomyscus mystax mystax, and Calomyscus sp. from Isfahan Province with 2n = 44 from karyotypes of C. bailwardi, Calomyscus sp. from Shahr-e-Kord, Chahar mahal and Bakhtiari-Aloni, and Khuzestan-Izeh Provinces with 2n = 46. The individuals from Shahdad tunnel, Kerman Province with 2n = 51–52 demonstrated non-centric fissions of chromosomes 4, 5, and 6 of the 46-chromosomal form with the formation of separate small acrocentrics. A heteromorphic pair of chromosomes in a specimen with 2n = 51 resulted from a fusion of two autosomes. C-banding and chromomycin A3-DAPI staining after G-banding showed extensive heterochromatin variation between individuals.

KW - Banding

KW - Chromosome painting

KW - Fluorescent in situ hybridization

KW - Karyotype

KW - Molecular cytogenetics

KW - Painting probes

KW - Type locality

UR - http://www.scopus.com/inward/record.url?scp=85109181623&partnerID=8YFLogxK

U2 - 10.3390/genes12070964

DO - 10.3390/genes12070964

M3 - Article

C2 - 34202749

AN - SCOPUS:85109181623

VL - 12

JO - Genes

JF - Genes

SN - 2073-4425

IS - 7

M1 - 964

ER -