TY - JOUR

T1 - Morphological Post-implantation Features of Aortic Conduits After Long-term wet Storage

AU - Sergeevichev, David

AU - Vasiliyeva, Maria

AU - Kuznetsova, Elena

AU - Zhulkov, Maksim

AU - Rusakova, Yanina

AU - Chepeleva, Elena

AU - Zhuravleva, Irina

N1 - Acknowledgements: This work was carried out within the state assignment of Ministry of Health of Russian Federation (theme № 121031300224-1).

PY - 2023/4

Y1 - 2023/4

N2 - Purpose: Improved methods for obtaining connective tissue structures in cardiac surgery are actively developed over the world. An important aspect of this research pertains to long-term wet storage of cardiovascular biomaterial that optimally preserves the initial morphological characteristics. Methods: Native and decellularized porcine aortas were stored for 50 days in several biocidal solutions (complex alcohol solution, a mixture of ethanol and glycerol, a mixture of antibiotics), and subsequently implanted in animals and studied histologically. The aortic specimens were implanted subcutaneously into 15 adult WAG rats. The samples were retrieved after 3 months and evaluated for calcium content using a quantitative spectroanalytical procedure. Aortic specimens were also orthotopically implanted into the vascular bed of 3 minipigs, harvested after 6 months, and histologically evaluated. Results: Atomic absorption spectroscopy showed that decellularized materials accumulated 4.8–9.2 times less calcium, depending on the storage solution used. Morphological and morphometrical analysis of orthotopically implanted aortic fragments in minipigs showed better preservation of decellularized material that had been stored in a complex alcohol solution and mixture of antibiotics. Conclusion: Functional and histological evidence was obtained demonstrating long-term storage without freezing of o aortic specimens without alteration of their biophysical properties and structure. The developed technique will be used in the future for the fabrication of vascular prostheses and heart valves.

AB - Purpose: Improved methods for obtaining connective tissue structures in cardiac surgery are actively developed over the world. An important aspect of this research pertains to long-term wet storage of cardiovascular biomaterial that optimally preserves the initial morphological characteristics. Methods: Native and decellularized porcine aortas were stored for 50 days in several biocidal solutions (complex alcohol solution, a mixture of ethanol and glycerol, a mixture of antibiotics), and subsequently implanted in animals and studied histologically. The aortic specimens were implanted subcutaneously into 15 adult WAG rats. The samples were retrieved after 3 months and evaluated for calcium content using a quantitative spectroanalytical procedure. Aortic specimens were also orthotopically implanted into the vascular bed of 3 minipigs, harvested after 6 months, and histologically evaluated. Results: Atomic absorption spectroscopy showed that decellularized materials accumulated 4.8–9.2 times less calcium, depending on the storage solution used. Morphological and morphometrical analysis of orthotopically implanted aortic fragments in minipigs showed better preservation of decellularized material that had been stored in a complex alcohol solution and mixture of antibiotics. Conclusion: Functional and histological evidence was obtained demonstrating long-term storage without freezing of o aortic specimens without alteration of their biophysical properties and structure. The developed technique will be used in the future for the fabrication of vascular prostheses and heart valves.

KW - Antimicrobial solution

KW - Cardiac prosthesis

KW - Connective tissue

KW - Decellularization

KW - Tissue mineralization

KW - Xenograft

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85149457628&origin=inward&txGid=62aa3ac1564efaeb030b715ee9b85e64

UR - https://elibrary.ru/item.asp?id=50738285

UR - https://www.mendeley.com/catalogue/bc1c6d64-e236-3efb-8693-f9cddc091efc/

U2 - 10.1007/s40846-023-00784-1

DO - 10.1007/s40846-023-00784-1

M3 - Article

VL - 43

SP - 185

EP - 194

JO - Journal of Medical and Biological Engineering

JF - Journal of Medical and Biological Engineering

SN - 2199-4757

IS - 2

ER -