TY - JOUR

T1 - Modular lentiviral vector system for chimeric antigen receptor design optimization

AU - Kulemzin, S. V.

AU - Chikaev, N. A.

AU - Volkova, O. Y.

AU - Kuznetsova, V. V.

AU - Taranin, A. V.

AU - Gorchakov, A. A.

N1 - Publisher Copyright: © 2017, Pleiades Publishing, Ltd.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The article reports the development of a collection of lentiviral vector constructs enabling time-efficient production and testing of different variants of chimeric antigen receptors (CAR). These artificial surface proteins make it possible to redirect the activity of immune cytotoxic T-cells towards cancer cells. Chimeric antigen receptors usually encompass four functional modules, namely, antigen recognition, flexible linker, transmembrane, and signal modules. The use of modules with different properties allows modulating the affinity and specificity of CAR interaction with target antigens, as well as intensity and quality of activation signaling, which determines the cytotoxic properties of CAR T-cells, as well as their proliferation rate and time of persistence in the organism. The proposed vector system make it possible to easily test various combinations of CAR modules while its being open to distribution allows the direct comparison of the results obtained by different scientific groups.

AB - The article reports the development of a collection of lentiviral vector constructs enabling time-efficient production and testing of different variants of chimeric antigen receptors (CAR). These artificial surface proteins make it possible to redirect the activity of immune cytotoxic T-cells towards cancer cells. Chimeric antigen receptors usually encompass four functional modules, namely, antigen recognition, flexible linker, transmembrane, and signal modules. The use of modules with different properties allows modulating the affinity and specificity of CAR interaction with target antigens, as well as intensity and quality of activation signaling, which determines the cytotoxic properties of CAR T-cells, as well as their proliferation rate and time of persistence in the organism. The proposed vector system make it possible to easily test various combinations of CAR modules while its being open to distribution allows the direct comparison of the results obtained by different scientific groups.

KW - chimeric antigen receptors

KW - lentiviral vector constructs

UR - http://www.scopus.com/inward/record.url?scp=85017613621&partnerID=8YFLogxK

U2 - 10.1134/S1068162017020091

DO - 10.1134/S1068162017020091

M3 - Article

AN - SCOPUS:85017613621

VL - 43

SP - 107

EP - 114

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 2

ER -