Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Modified Oligonucleotides for Guiding RNA Cleavage Using Bacterial RNase P. / Novopashina, D. S.; Nazarov, A. S.; Vorobjeva, M. A. и др.
в: Molekuliarnaia biologiia, Том 52, № 6, 01.11.2018, стр. 1045-1054.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Modified Oligonucleotides for Guiding RNA Cleavage Using Bacterial RNase P
AU - Novopashina, D. S.
AU - Nazarov, A. S.
AU - Vorobjeva, M. A.
AU - Kuprushkin, M. S.
AU - Davydova, A. S.
AU - Lomzov, A. A.
AU - Pyshnyi, D. V.
AU - Altman, S.
AU - Venyaminova, A. G.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - The ability of a series of novel modified external guide sequences (EGS oligonucleotides) to induce the hydrolysis of target RNA with bacterial ribonuclease P has been studied; the most efficient modification variants have been selected. We have found patterns of the oligonucleotide sugar-phosphate backbone modi-fications that enhance oligonucleotide stability in the biological environment and do not violate the ability to interact with the enzyme and induce the RNA hydrolysis. It has been shown that analogues of EGS oligonucleotides selectively modified at 2'-position (2'-O-methyl and 2'-fluoro) or at internucleotide phosphates (phosphoryl guanidines) can be used for the addressed cleavage of a model RNA target by bacterial RNase P. The ability of new phosphoryl guanidine analogues of oligodeoxyribonucleotides that are stable in biological media to induce the hydrolysis of target RNA with bacterial ribonuclease P has been shown for the first time. The modified EGS oligonucleotides with an optimal balance between functional activity and stability in biological media can be considered as potential antibacterial agents.
AB - The ability of a series of novel modified external guide sequences (EGS oligonucleotides) to induce the hydrolysis of target RNA with bacterial ribonuclease P has been studied; the most efficient modification variants have been selected. We have found patterns of the oligonucleotide sugar-phosphate backbone modi-fications that enhance oligonucleotide stability in the biological environment and do not violate the ability to interact with the enzyme and induce the RNA hydrolysis. It has been shown that analogues of EGS oligonucleotides selectively modified at 2'-position (2'-O-methyl and 2'-fluoro) or at internucleotide phosphates (phosphoryl guanidines) can be used for the addressed cleavage of a model RNA target by bacterial RNase P. The ability of new phosphoryl guanidine analogues of oligodeoxyribonucleotides that are stable in biological media to induce the hydrolysis of target RNA with bacterial ribonuclease P has been shown for the first time. The modified EGS oligonucleotides with an optimal balance between functional activity and stability in biological media can be considered as potential antibacterial agents.
KW - 2'-fluoro modified oligoribonucleotides
KW - bacterial RNase P
KW - EGS oligonucleotides
KW - modified oligonucleotides
KW - oligo(2'-О-methylribonucleotides)
KW - phosphoryl guanidine oligonucleotides
KW - Bacteria
KW - Oligonucleotides/chemistry
KW - RNA/chemistry
KW - RNA Cleavage
KW - Ribonuclease P/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85059828756&partnerID=8YFLogxK
U2 - 10.1134/S0026898418060137
DO - 10.1134/S0026898418060137
M3 - Article
C2 - 30633247
AN - SCOPUS:85059828756
VL - 52
SP - 1045
EP - 1054
JO - Molekulyarnaya Biologiya
JF - Molekulyarnaya Biologiya
SN - 0026-8984
IS - 6
ER -
ID: 18119413