TY - CONF

T1 - Mild phenotype of knockouts of the major apurinic/apyrimidinic endonuclease APEX1 in a noncancer human cell line

AU - Ким, Дарья Вячеславовна

AU - Кулишова, Лилия Михайловна

AU - Торгашева, Наталья Александровна

AU - Жарков, Дмитрий Олегович

N1 - This research was supported by RFBR (grant 20-34-90092).

PY - 2021/8

Y1 - 2021/8

N2 - A well-known approach to investigate protein functions is to generate knockout animals or cell lines. In the case of many base excision repair (BER) proteins (APEX1, POLB, LIG3, XRCC1) these knockout mice die at the early stages of embryonic development. The CRISPR/Cas9 genome editing technology offers a new way to generate knockouts without causing profound gene disruption. Using this technology, we have established human cell lines deficient in the major apurinic/apyrimidinic (AP) endonuclease, APEX1.

AB - A well-known approach to investigate protein functions is to generate knockout animals or cell lines. In the case of many base excision repair (BER) proteins (APEX1, POLB, LIG3, XRCC1) these knockout mice die at the early stages of embryonic development. The CRISPR/Cas9 genome editing technology offers a new way to generate knockouts without causing profound gene disruption. Using this technology, we have established human cell lines deficient in the major apurinic/apyrimidinic (AP) endonuclease, APEX1.

M3 - Abstract

SP - 92

T2 - GENOME ENGINEERING: CRISPR FRONTIERS. Virtual meeting

Y2 - 18 August 2021 through 20 August 2021

ER -