TY - JOUR

T1 - Microorganisms of two thermal pools on kunashir island, russia

AU - Rozanov, Aleksei S.

AU - Korzhuk, Anton V.

AU - Shekhovtsov, Sergei V.

AU - Vasiliev, Gennady V.

AU - Peltek, Sergei E.

N1 - Funding Information: Funding: This study was supported by Kurchatov Genomics Center IC&G (№075–15–2019–1662). We thank the Collection microorganisms of “Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences” (supported by the Ministry of Science and Higher Education project 0259-2019-0005). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/9

Y1 - 2021/9

N2 - The Kuril Archipelago is a part of the Circum-Pacific Belt (Ring of Fire). These islands have numerous thermal springs. There are very few studies on these microbial communities, and none of them have been conducted by modern molecular biological methods. Here we performed the first metagenomic study on two thermophilic microbial communities of Kunashir Island. Faust Lake is hot (48◦C) and highly acidic (pH 2.0). We constructed 28 metagenome-assembled genomes as well as 17 16S ribosomal RNA sequences. We found that bottom sediments of Faust Lake are dominated by a single species of red algae belonging to the Cyanidiaceae family. Archaeans in Faust Lake are more diverse than bacteria but less abundant. The Tretyakovsky Thermal Spring is also hot (52◦C) but only weakly acidic (pH 6.0). It has much higher microbial diversity (233 metagenome-assembled genomes; 93 16S ribosomal RNAs) and is dominated by bacteria, with only several archaeans and one fungus. Despite their geographic proximity, these two thermal springs were found to not share any species. A comparison of these two lakes with other thermal springs of the Circum-Pacific Belt revealed that only a few members of the communities are shared among different locations.

AB - The Kuril Archipelago is a part of the Circum-Pacific Belt (Ring of Fire). These islands have numerous thermal springs. There are very few studies on these microbial communities, and none of them have been conducted by modern molecular biological methods. Here we performed the first metagenomic study on two thermophilic microbial communities of Kunashir Island. Faust Lake is hot (48◦C) and highly acidic (pH 2.0). We constructed 28 metagenome-assembled genomes as well as 17 16S ribosomal RNA sequences. We found that bottom sediments of Faust Lake are dominated by a single species of red algae belonging to the Cyanidiaceae family. Archaeans in Faust Lake are more diverse than bacteria but less abundant. The Tretyakovsky Thermal Spring is also hot (52◦C) but only weakly acidic (pH 6.0). It has much higher microbial diversity (233 metagenome-assembled genomes; 93 16S ribosomal RNAs) and is dominated by bacteria, with only several archaeans and one fungus. Despite their geographic proximity, these two thermal springs were found to not share any species. A comparison of these two lakes with other thermal springs of the Circum-Pacific Belt revealed that only a few members of the communities are shared among different locations.

KW - Genome

KW - Hot spring

KW - Kuril islands

KW - Metagenome

KW - Microbial community

UR - http://www.scopus.com/inward/record.url?scp=85115267740&partnerID=8YFLogxK

U2 - 10.3390/biology10090924

DO - 10.3390/biology10090924

M3 - Article

C2 - 34571800

AN - SCOPUS:85115267740

VL - 10

JO - Biology

JF - Biology

SN - 2079-7737

IS - 9

M1 - 924

ER -