Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Metabolomics of the rat lens: A combined LC-MS and NMR study. / Yanshole, Vadim V.; Snytnikova, Olga A.; Kiryutin, Alexey S. и др.
в: Experimental eye research, Том 125, 08.2014, стр. 71-78.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Metabolomics of the rat lens: A combined LC-MS and NMR study
AU - Yanshole, Vadim V.
AU - Snytnikova, Olga A.
AU - Kiryutin, Alexey S.
AU - Yanshole, Lyudmila V.
AU - Sagdeev, Renad Z.
AU - Tsentalovich, Yuri P.
N1 - Funding Information: We thank Prof. N.G. Kolosova and Dr. N.A. Stefanova for providing the experimental animals. This work was supported by RFBR (Projects 14-03-00027, 14-03-00453 and 14-03-31189), by the Ministry of Education and Science RF, and by the Division of Chemistry of RAS.
PY - 2014/8
Y1 - 2014/8
N2 - This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine, lactate, phosphocholine and reduced glutathione. The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan, tyrosine, carnitine, glycerophosphate, GSH and GSSG were found in lenses of senescence-accelerated OXYS rats; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress.
AB - This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine, lactate, phosphocholine and reduced glutathione. The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan, tyrosine, carnitine, glycerophosphate, GSH and GSSG were found in lenses of senescence-accelerated OXYS rats; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress.
KW - Cataract
KW - Lens
KW - Metabolomics
UR - http://www.scopus.com/inward/record.url?scp=84902492445&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=24059534
U2 - 10.1016/j.exer.2014.05.016
DO - 10.1016/j.exer.2014.05.016
M3 - Article
C2 - 24910091
AN - SCOPUS:84902492445
VL - 125
SP - 71
EP - 78
JO - Experimental eye research
JF - Experimental eye research
SN - 0014-4835
ER -
ID: 34440729