Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Metabolomics of the human aqueous humor. / Snytnikova, Olga A.; Khlichkina, Anastasiya A.; Yanshole, Lyudmila V. и др.
в: Metabolomics, Том 13, № 1, 5, 01.01.2017.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Metabolomics of the human aqueous humor
AU - Snytnikova, Olga A.
AU - Khlichkina, Anastasiya A.
AU - Yanshole, Lyudmila V.
AU - Yanshole, Vadim V.
AU - Iskakov, Igor A.
AU - Egorova, Elena V.
AU - Stepakov, Denis A.
AU - Novoselov, Vladimir P.
AU - Tsentalovich, Yuri P.
N1 - Publisher Copyright: © 2016, Springer Science+Business Media New York.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Introduction: The optical elements of the eye—cornea, lens, and vitreous humor—are avascular tissues, and their nutrition and waste removal are provided by aqueous humor (AH). The AH production occurs through the active secretion and the passive diffusion/ultrafiltration of blood plasma. The comparison of the metabolomic profiles of AH and plasma is important for understanding of the mechanisms of biochemical processes and metabolite transport taking place in vivo in ocular tissues. Objectives: The work is aimed at the determination of concentrations of a wide range of most abundant metabolites in the human AH, the comparison of the metabolomic profiles of AH and serum, and the analysis of the post-mortem metabolomic changes in these two biological fluids. Methods: The quantitative metabolomic profiling was carried out with the use of two independent methods—high-frequency 1H NMR spectroscopy and HPLC with high-resolution ESI-MS detection. Results: The concentrations of 71 most abundant metabolites in blood serum and AH from living patients and human cadavers have been measured. It has been found that the level of ascorbate in AH is by two orders of magnitude higher than that in serum; the levels of other metabolites are either similar to that in serum, or differ from that by a factor of 2–5. The post-mortem metabolomic composition of both serum and AH undergoes rapid and strong changes. Conclusion: The differences between the metabolomic profiles of AH and serum for majority of metabolites can be attributed to the metabolic activity of the ocular tissues leading to the lack or excess of some metabolites, while the high concentration of ascorbate in AH demonstrates the activity of ascorbate-specific pumps at the blood-aqueous border. The post-mortem metabolomic changes are caused by the disruption of the major biochemical cycles and cell lysis. These changes should be taken into account in the analysis of disease-induced changes in post-mortem samples of the ocular tissues.
AB - Introduction: The optical elements of the eye—cornea, lens, and vitreous humor—are avascular tissues, and their nutrition and waste removal are provided by aqueous humor (AH). The AH production occurs through the active secretion and the passive diffusion/ultrafiltration of blood plasma. The comparison of the metabolomic profiles of AH and plasma is important for understanding of the mechanisms of biochemical processes and metabolite transport taking place in vivo in ocular tissues. Objectives: The work is aimed at the determination of concentrations of a wide range of most abundant metabolites in the human AH, the comparison of the metabolomic profiles of AH and serum, and the analysis of the post-mortem metabolomic changes in these two biological fluids. Methods: The quantitative metabolomic profiling was carried out with the use of two independent methods—high-frequency 1H NMR spectroscopy and HPLC with high-resolution ESI-MS detection. Results: The concentrations of 71 most abundant metabolites in blood serum and AH from living patients and human cadavers have been measured. It has been found that the level of ascorbate in AH is by two orders of magnitude higher than that in serum; the levels of other metabolites are either similar to that in serum, or differ from that by a factor of 2–5. The post-mortem metabolomic composition of both serum and AH undergoes rapid and strong changes. Conclusion: The differences between the metabolomic profiles of AH and serum for majority of metabolites can be attributed to the metabolic activity of the ocular tissues leading to the lack or excess of some metabolites, while the high concentration of ascorbate in AH demonstrates the activity of ascorbate-specific pumps at the blood-aqueous border. The post-mortem metabolomic changes are caused by the disruption of the major biochemical cycles and cell lysis. These changes should be taken into account in the analysis of disease-induced changes in post-mortem samples of the ocular tissues.
KW - Aqueous humor
KW - Human metabolomics
KW - Serum
KW - RESOLUTION H-1-NMR SPECTROSCOPY
KW - RAT
KW - NMR-SPECTROSCOPY
KW - ASCORBIC-ACID
KW - POSTMORTEM INTERVAL
KW - VITREOUS HUMORS
KW - AMINO-ACIDS
KW - RABBIT
KW - LENS
KW - BLOOD
UR - http://www.scopus.com/inward/record.url?scp=85000785104&partnerID=8YFLogxK
U2 - 10.1007/s11306-016-1144-0
DO - 10.1007/s11306-016-1144-0
M3 - Article
AN - SCOPUS:85000785104
VL - 13
JO - Metabolomics
JF - Metabolomics
SN - 1573-3882
IS - 1
M1 - 5
ER -
ID: 10318311