Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Metabolomic composition of normal aged and cataractous human lenses. / Tsentalovich, Yuri P.; Verkhovod, Timofey D.; Yanshole, Vadim V. и др.
в: Experimental eye research, Том 134, 01.05.2015, стр. 15-23.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Metabolomic composition of normal aged and cataractous human lenses
AU - Tsentalovich, Yuri P.
AU - Verkhovod, Timofey D.
AU - Yanshole, Vadim V.
AU - Kiryutin, Alexey S.
AU - Yanshole, Lyudmila V.
AU - Fursova, Anjella Zh
AU - Stepakov, Denis A.
AU - Novoselov, Vladimir P.
AU - Sagdeev, Renad Z.
N1 - Funding Information: This work was supported by the Russian Scientific Foundation (project №. 14-14-00056 ). The NMR measurements were performed with the financial support from RFBR (project №. 14-03-00027 ) and the President of RF (project MK-5367.2015.3). Publisher Copyright: © 2015 Elsevier Ltd.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Quantitative metabolomic profiles of normal and cataractous human lenses were obtained with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. The concentration of more than fifty metabolites in the lens cortex and nucleus has been determined. For the majority of metabolites, their concentrations in the lens cortex and nucleus are similar, which confirms low metabolic activity in the lens core. The difference between the metabolite levels in the cortex and nucleus of the normal lens is observed for antioxidants and UV filters, which demonstrates the activity of redox processes in the lens. A huge difference is found between the metabolomic compositions of normal and age-matched cataractous lenses: the concentrations of almost all metabolites in the normal lens are higher than in the cataractous one. The most pronounced difference is observed for compounds playing a key role in the lens cell protection and metabolic activity, including antioxidants, UV filters, and osmolytes. The results obtained imply that the development of the age-related cataracts might originate from the metabolic dysfunction of the lens epithelial cells.
AB - Quantitative metabolomic profiles of normal and cataractous human lenses were obtained with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. The concentration of more than fifty metabolites in the lens cortex and nucleus has been determined. For the majority of metabolites, their concentrations in the lens cortex and nucleus are similar, which confirms low metabolic activity in the lens core. The difference between the metabolite levels in the cortex and nucleus of the normal lens is observed for antioxidants and UV filters, which demonstrates the activity of redox processes in the lens. A huge difference is found between the metabolomic compositions of normal and age-matched cataractous lenses: the concentrations of almost all metabolites in the normal lens are higher than in the cataractous one. The most pronounced difference is observed for compounds playing a key role in the lens cell protection and metabolic activity, including antioxidants, UV filters, and osmolytes. The results obtained imply that the development of the age-related cataracts might originate from the metabolic dysfunction of the lens epithelial cells.
KW - Aging
KW - Antioxidants
KW - Cataract
KW - Lens
KW - Metabolomics
KW - UV filters
UR - http://www.scopus.com/inward/record.url?scp=84928313599&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=24024458
U2 - 10.1016/j.exer.2015.03.008
DO - 10.1016/j.exer.2015.03.008
M3 - Article
C2 - 25773987
AN - SCOPUS:84928313599
VL - 134
SP - 15
EP - 23
JO - Experimental eye research
JF - Experimental eye research
SN - 0014-4835
ER -
ID: 34440959