Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Metabolomic Analysis Reveals That the Moor Frog Rana arvalis Uses Both Glucose and Glycerol as Cryoprotectants. / Shekhovtsov, Sergei V.; Bulakhova, Nina A.; Tsentalovich, Yuri P. и др.
в: Animals, Том 12, № 10, 1286, 01.05.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Metabolomic Analysis Reveals That the Moor Frog Rana arvalis Uses Both Glucose and Glycerol as Cryoprotectants
AU - Shekhovtsov, Sergei V.
AU - Bulakhova, Nina A.
AU - Tsentalovich, Yuri P.
AU - Zelentsova, Ekaterina A.
AU - Meshcheryakova, Ekaterina N.
AU - Poluboyarova, Tatiana V.
AU - Berman, Daniil I.
N1 - Funding Information: Funding: This study (animal collection and metabolomic analysis) was supported by the Russian Science Foundation (RSF) grant no. 21-74-20050. Animal laboratory handling was supported by State Budget Projects nos. 1021060307698-5 and FWNR-2022-0022. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5/1
Y1 - 2022/5/1
N2 - The moor frog Rana arvalis is one of a few amphibians that can tolerate freezing to low temperatures, up to −16 °C. In this study, we performed metabolomic analysis of the liver and hindlimb muscles of frozen and control R. arvalis. We found that the moor frog synthesizes glucose and glycerol in similar concentrations as low molecular weight cryoprotectants. This is the first such case reported for the genus Rana, which was believed to use glucose only. We found that freezing upregulates glycolysis, with the accumulation of several end products: lactate, alanine, ethanol, and, possibly, 2,3-butanediol. To our knowledge, this is also the first report of ethanol as an end product of glycolysis in terrestrial vertebrates. We observed highly increased concentrations of nucleotide degradation products, implying high level of stress. The Krebs cycle arrest resulted in high concentrations of succinate, which is common for animals. However, we found almost no signs of adaptations to reoxygenation stress, with overall low levels of antioxidants. We also performed metabo-lomics analysis of subcutaneous ice that was found to contain glucose, glycerol, and several other substances.
AB - The moor frog Rana arvalis is one of a few amphibians that can tolerate freezing to low temperatures, up to −16 °C. In this study, we performed metabolomic analysis of the liver and hindlimb muscles of frozen and control R. arvalis. We found that the moor frog synthesizes glucose and glycerol in similar concentrations as low molecular weight cryoprotectants. This is the first such case reported for the genus Rana, which was believed to use glucose only. We found that freezing upregulates glycolysis, with the accumulation of several end products: lactate, alanine, ethanol, and, possibly, 2,3-butanediol. To our knowledge, this is also the first report of ethanol as an end product of glycolysis in terrestrial vertebrates. We observed highly increased concentrations of nucleotide degradation products, implying high level of stress. The Krebs cycle arrest resulted in high concentrations of succinate, which is common for animals. However, we found almost no signs of adaptations to reoxygenation stress, with overall low levels of antioxidants. We also performed metabo-lomics analysis of subcutaneous ice that was found to contain glucose, glycerol, and several other substances.
KW - cryoprotectants
KW - freezing tolerance
KW - metabolomics analysis
KW - Rana arvalis
UR - http://www.scopus.com/inward/record.url?scp=85130174693&partnerID=8YFLogxK
U2 - 10.3390/ani12101286
DO - 10.3390/ani12101286
M3 - Article
C2 - 35625132
AN - SCOPUS:85130174693
VL - 12
JO - Animals
JF - Animals
SN - 2076-2615
IS - 10
M1 - 1286
ER -
ID: 36565084