Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Membrane-Sugar Interactions Probed by Low-Frequency Raman Spectroscopy : The Monolayer Adsorption Model. / Leonov, Dmitry V.; Dzuba, Sergei A.; Surovtsev, Nikolay V.
в: Langmuir : the ACS journal of surfaces and colloids, Том 36, № 39, 06.10.2020, стр. 11655-11660.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Membrane-Sugar Interactions Probed by Low-Frequency Raman Spectroscopy
T2 - The Monolayer Adsorption Model
AU - Leonov, Dmitry V.
AU - Dzuba, Sergei A.
AU - Surovtsev, Nikolay V.
N1 - Publisher Copyright: © 2020 American Chemical Society. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10/6
Y1 - 2020/10/6
N2 - Small sugars are known to stabilize biological membranes under extreme conditions of freezing and desiccation. The proposed mechanisms of stabilization suggest membrane-sugar interactions to be either attractive or repulsive. To obtain new insight into the problem, we use a recently developed low-frequency Raman scattering approach which allows detecting membrane mechanical vibrations. For model membranes of palmitoyl-oleoyl-glycero-phosphocholine (POPC) hydrated in aqueous sucrose and trehalose solutions, we studied the Raman peak between 12 and 15 cm-1 that is attributed to an eigenmode of the normal mechanical vibrations of a lipid monolayer. For both sugars, similar results were obtained. With an increase in sugar concentration in solution, the frequency position of the peak was found to decrease by ∼13% which was interpreted as a consequence of the membrane thickening due sugar monolayer adsorption on the membrane surface. The concentration dependence of the peak frequency position was satisfactorily described by a Langmuir monolayer adsorption model. It is concluded that, at small sugar concentrations (less than 0.2 M), the membrane-sugar interactions are attractive, while at higher concentrations (more than 0.4 M) the attraction disappears. The data obtained show that one sugar molecule on the surface interacts with approximately 3-4 polar lipid heads.
AB - Small sugars are known to stabilize biological membranes under extreme conditions of freezing and desiccation. The proposed mechanisms of stabilization suggest membrane-sugar interactions to be either attractive or repulsive. To obtain new insight into the problem, we use a recently developed low-frequency Raman scattering approach which allows detecting membrane mechanical vibrations. For model membranes of palmitoyl-oleoyl-glycero-phosphocholine (POPC) hydrated in aqueous sucrose and trehalose solutions, we studied the Raman peak between 12 and 15 cm-1 that is attributed to an eigenmode of the normal mechanical vibrations of a lipid monolayer. For both sugars, similar results were obtained. With an increase in sugar concentration in solution, the frequency position of the peak was found to decrease by ∼13% which was interpreted as a consequence of the membrane thickening due sugar monolayer adsorption on the membrane surface. The concentration dependence of the peak frequency position was satisfactorily described by a Langmuir monolayer adsorption model. It is concluded that, at small sugar concentrations (less than 0.2 M), the membrane-sugar interactions are attractive, while at higher concentrations (more than 0.4 M) the attraction disappears. The data obtained show that one sugar molecule on the surface interacts with approximately 3-4 polar lipid heads.
KW - MOLECULAR-DYNAMICS SIMULATIONS
KW - LIPID-BILAYERS
KW - PHASE-SEPARATION
KW - TREHALOSE
KW - SUCROSE
KW - POPC
KW - MECHANISMS
KW - HYDRATION
KW - DPPC
UR - http://www.scopus.com/inward/record.url?scp=85092681537&partnerID=8YFLogxK
U2 - 10.1021/acs.langmuir.0c02458
DO - 10.1021/acs.langmuir.0c02458
M3 - Article
C2 - 32975956
AN - SCOPUS:85092681537
VL - 36
SP - 11655
EP - 11660
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 39
ER -
ID: 25645017