TY - JOUR

T1 - Male Meiotic Recombination in the Steppe Agama, Trapelus sanguinolentus (Agamidae, Iguania, Reptilia)

AU - Lisachov, Artem P.

AU - Tishakova, Katerina V.

AU - Tsepilov, Yakov A.

AU - Borodin, Pavel M.

N1 - © 2019 S. Karger AG, Basel.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Meiotic recombination rates and patterns of crossover distributions along the chromosomes vary considerably even between closely related species. The adaptive significance of these differences is still unclear due to the paucity of empirical data. Most data on recombination come from mammalian species, while other vertebrate clades are poorly explored. Using immunolocalization of the protein of the lateral element of the synaptonemal complex (SYCP3) and the mismatch-repair protein MLH1, which marks mature recombination nodules, we analyzed recombination rates and crossover distribution in meiotic prophase chromosomes of the steppe agama (Trapelus sanguinolentus, Agamidae, Acrodonta, Iguania) and compared them with data obtained for the genus Anolis (Dactyloidae, Pleurodonta, Iguania). We found that, despite a smaller genome size, the total SC length and the MLH1 focus number per cell are much higher in the agama than in the anoles. The distributions of the MLH1 foci in the agama are multimodal in larger chromosomes and bimodal in smaller chromosomes without a significant centromere effect, resembling the patterns known for birds. A possible relationship between karyotype remodeling and the evolution of recombination in Iguania is discussed.

AB - Meiotic recombination rates and patterns of crossover distributions along the chromosomes vary considerably even between closely related species. The adaptive significance of these differences is still unclear due to the paucity of empirical data. Most data on recombination come from mammalian species, while other vertebrate clades are poorly explored. Using immunolocalization of the protein of the lateral element of the synaptonemal complex (SYCP3) and the mismatch-repair protein MLH1, which marks mature recombination nodules, we analyzed recombination rates and crossover distribution in meiotic prophase chromosomes of the steppe agama (Trapelus sanguinolentus, Agamidae, Acrodonta, Iguania) and compared them with data obtained for the genus Anolis (Dactyloidae, Pleurodonta, Iguania). We found that, despite a smaller genome size, the total SC length and the MLH1 focus number per cell are much higher in the agama than in the anoles. The distributions of the MLH1 foci in the agama are multimodal in larger chromosomes and bimodal in smaller chromosomes without a significant centromere effect, resembling the patterns known for birds. A possible relationship between karyotype remodeling and the evolution of recombination in Iguania is discussed.

KW - Immunolocalization

KW - Meiotic recombination

KW - MLH1 foci

KW - Steppe agama

KW - LIZARDS

KW - SEX

KW - CHROMOSOMES

KW - GENOME

KW - EVOLUTION

KW - MACROCHROMOSOMES

KW - SYNAPTONEMAL COMPLEX LENGTH

KW - SQUAMATA

KW - CROSSING-OVER

KW - Meiosis/genetics

KW - Synaptonemal Complex/genetics

KW - Species Specificity

KW - Homologous Recombination

KW - Male

KW - Centromere/genetics

KW - Crossing Over, Genetic/genetics

KW - Lizards/classification

KW - Animals

KW - Genome Size

KW - MutL Protein Homolog 1/genetics

KW - Karyotype

UR - http://www.scopus.com/inward/record.url?scp=85060603484&partnerID=8YFLogxK

U2 - 10.1159/000496078

DO - 10.1159/000496078

M3 - Article

C2 - 30677759

AN - SCOPUS:85060603484

VL - 157

SP - 107

EP - 114

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 1-2

ER -