Результаты исследований: Научные публикации в периодических изданиях › обзорная статья › Рецензирование
Main Strategies of Plant Expression System Glycoengineering for Producing Humanized Recombinant Pharmaceutical Proteins. / Rozov, S. M.; Permyakova, N. V.; Deineko, E. V.
в: Biochemistry (Moscow), Том 83, № 3, 01.03.2018, стр. 215-232.Результаты исследований: Научные публикации в периодических изданиях › обзорная статья › Рецензирование
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TY - JOUR
T1 - Main Strategies of Plant Expression System Glycoengineering for Producing Humanized Recombinant Pharmaceutical Proteins
AU - Rozov, S. M.
AU - Permyakova, N. V.
AU - Deineko, E. V.
N1 - Publisher Copyright: © 2018, Pleiades Publishing, Ltd. Copyright: Copyright 2018 Elsevier B.V., All rights reserved. Publisher Copyright: © 2018, Pleiades Publishing, Ltd.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Most the pharmaceutical proteins are derived not from their natural sources, rather their recombinant analogs are synthesized in various expression systems. Plant expression systems, unlike mammalian cell cultures, combine simplicity and low cost of procaryotic systems and the ability for posttranslational modifications inherent in eucaryotes. More than 50% of all human proteins and more than 40% of the currently used pharmaceutical proteins are glycosylated, that is, they are glycoproteins, and their biological activity, pharmacodynamics, and immunogenicity depend on the correct glycosylation pattern. This review examines in detail the similarities and differences between N- and O–glycosylation in plant and mammalian cells, as well as the effect of plant glycans on the activity, pharmacokinetics, immunity, and intensity of biosynthesis of pharmaceutical proteins. The main current strategies of glycoengineering of plant expression systems aimed at obtaining fully humanized proteins for pharmaceutical application are summarized.
AB - Most the pharmaceutical proteins are derived not from their natural sources, rather their recombinant analogs are synthesized in various expression systems. Plant expression systems, unlike mammalian cell cultures, combine simplicity and low cost of procaryotic systems and the ability for posttranslational modifications inherent in eucaryotes. More than 50% of all human proteins and more than 40% of the currently used pharmaceutical proteins are glycosylated, that is, they are glycoproteins, and their biological activity, pharmacodynamics, and immunogenicity depend on the correct glycosylation pattern. This review examines in detail the similarities and differences between N- and O–glycosylation in plant and mammalian cells, as well as the effect of plant glycans on the activity, pharmacokinetics, immunity, and intensity of biosynthesis of pharmaceutical proteins. The main current strategies of glycoengineering of plant expression systems aimed at obtaining fully humanized proteins for pharmaceutical application are summarized.
KW - glycoengineering
KW - glycosyltransferases
KW - pharmaceutical proteins
KW - plant expression systems
KW - protein glycosylation
UR - http://www.scopus.com/inward/record.url?scp=85044237770&partnerID=8YFLogxK
U2 - 10.1134/S0006297918030033
DO - 10.1134/S0006297918030033
M3 - Review article
C2 - 29625542
AN - SCOPUS:85044237770
VL - 83
SP - 215
EP - 232
JO - Biochemistry (Moscow)
JF - Biochemistry (Moscow)
SN - 0006-2979
IS - 3
ER -
ID: 12178950