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Localization of the ibuprofen molecule in model lipid membranes revealed by spin-label-enhanced NMR relaxation. / Kashnik, Anna S; Selyutina, Olga Yu; Baranov, Denis S и др.

в: Biochimica et Biophysica Acta - Biomembranes, Том 1865, № 8, 184215, 12.2023.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Kashnik, AS, Selyutina, OY, Baranov, DS, Polyakov, NE & Dzuba, SA 2023, 'Localization of the ibuprofen molecule in model lipid membranes revealed by spin-label-enhanced NMR relaxation', Biochimica et Biophysica Acta - Biomembranes, Том. 1865, № 8, 184215. https://doi.org/10.1016/j.bbamem.2023.184215

APA

Kashnik, A. S., Selyutina, O. Y., Baranov, D. S., Polyakov, N. E., & Dzuba, S. A. (2023). Localization of the ibuprofen molecule in model lipid membranes revealed by spin-label-enhanced NMR relaxation. Biochimica et Biophysica Acta - Biomembranes, 1865(8), [184215]. https://doi.org/10.1016/j.bbamem.2023.184215

Vancouver

Kashnik AS, Selyutina OY, Baranov DS, Polyakov NE, Dzuba SA. Localization of the ibuprofen molecule in model lipid membranes revealed by spin-label-enhanced NMR relaxation. Biochimica et Biophysica Acta - Biomembranes. 2023 дек.;1865(8):184215. doi: 10.1016/j.bbamem.2023.184215

Author

Kashnik, Anna S ; Selyutina, Olga Yu ; Baranov, Denis S и др. / Localization of the ibuprofen molecule in model lipid membranes revealed by spin-label-enhanced NMR relaxation. в: Biochimica et Biophysica Acta - Biomembranes. 2023 ; Том 1865, № 8.

BibTeX

@article{fb1b946f2dd5433aad8043032f140386,
title = "Localization of the ibuprofen molecule in model lipid membranes revealed by spin-label-enhanced NMR relaxation",
abstract = "Non-steroidal anti-inflammatory drugs (NSAIDs) have antipyretic, anti-inflammatory and analgesic effects, and can be used in the treatment of various diseases. These drugs have also a number of side effects, which may be related to their interaction with lipid membranes. In this study, we use the spin-labeled NSAID ibuprofen (ibuprofen-SL) as a relaxation enhancer to study its interaction with model lipid membranes employing liquid-state 1H NMR at 500 MHz. The high magnetic moment of unpaired electron in the spin label made it possible to reduce the concentration of the studied drug in the membrane to tenths of a mole percent. As model membranes, unilamellar POPC liposomes and bicelles consisting of a 2:1 mixture of DHPC:DMPC or DHPC:POPC lipids were used. An increase in the rate of proton spin-lattice relaxation, T1-1, selectively detected for protons at different positions in the lipid molecule, showed that ibuprofen-SL is localized in the hydrophobic part of the lipid bilayer. As the concentration of ibuprofen-SL increases to 0.5 mol%, the distribution of positions of ibuprofen-SL across the bilayer becomes wider. In the presence of 20 mol% of cholesterol, ibuprofen-SL is displaced from the core of the membrane to a region closer to the head group of the bilayer. This displacement was also confirmed by the NMR NOESY experiment conducted with unlabeled ibuprofen. For bilayers containing unsaturated POPC lipids, the distribution of ibuprofen positions across the bilayer becomes narrower compared to the presence of saturated DMPC lipids.",
keywords = "Ibuprofen, Dimyristoylphosphatidylcholine, Anti-Inflammatory Agents, Non-Steroidal, Electrons",
author = "Kashnik, {Anna S} and Selyutina, {Olga Yu} and Baranov, {Denis S} and Polyakov, {Nikolay E} and Dzuba, {Sergei A}",
note = "This work was supported by the Russian Science Foundation, project # 21-13-00025. Copyright {\textcopyright} 2023 Elsevier B.V. All rights reserved.",
year = "2023",
month = dec,
doi = "10.1016/j.bbamem.2023.184215",
language = "English",
volume = "1865",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "8",

}

RIS

TY - JOUR

T1 - Localization of the ibuprofen molecule in model lipid membranes revealed by spin-label-enhanced NMR relaxation

AU - Kashnik, Anna S

AU - Selyutina, Olga Yu

AU - Baranov, Denis S

AU - Polyakov, Nikolay E

AU - Dzuba, Sergei A

N1 - This work was supported by the Russian Science Foundation, project # 21-13-00025. Copyright © 2023 Elsevier B.V. All rights reserved.

PY - 2023/12

Y1 - 2023/12

N2 - Non-steroidal anti-inflammatory drugs (NSAIDs) have antipyretic, anti-inflammatory and analgesic effects, and can be used in the treatment of various diseases. These drugs have also a number of side effects, which may be related to their interaction with lipid membranes. In this study, we use the spin-labeled NSAID ibuprofen (ibuprofen-SL) as a relaxation enhancer to study its interaction with model lipid membranes employing liquid-state 1H NMR at 500 MHz. The high magnetic moment of unpaired electron in the spin label made it possible to reduce the concentration of the studied drug in the membrane to tenths of a mole percent. As model membranes, unilamellar POPC liposomes and bicelles consisting of a 2:1 mixture of DHPC:DMPC or DHPC:POPC lipids were used. An increase in the rate of proton spin-lattice relaxation, T1-1, selectively detected for protons at different positions in the lipid molecule, showed that ibuprofen-SL is localized in the hydrophobic part of the lipid bilayer. As the concentration of ibuprofen-SL increases to 0.5 mol%, the distribution of positions of ibuprofen-SL across the bilayer becomes wider. In the presence of 20 mol% of cholesterol, ibuprofen-SL is displaced from the core of the membrane to a region closer to the head group of the bilayer. This displacement was also confirmed by the NMR NOESY experiment conducted with unlabeled ibuprofen. For bilayers containing unsaturated POPC lipids, the distribution of ibuprofen positions across the bilayer becomes narrower compared to the presence of saturated DMPC lipids.

AB - Non-steroidal anti-inflammatory drugs (NSAIDs) have antipyretic, anti-inflammatory and analgesic effects, and can be used in the treatment of various diseases. These drugs have also a number of side effects, which may be related to their interaction with lipid membranes. In this study, we use the spin-labeled NSAID ibuprofen (ibuprofen-SL) as a relaxation enhancer to study its interaction with model lipid membranes employing liquid-state 1H NMR at 500 MHz. The high magnetic moment of unpaired electron in the spin label made it possible to reduce the concentration of the studied drug in the membrane to tenths of a mole percent. As model membranes, unilamellar POPC liposomes and bicelles consisting of a 2:1 mixture of DHPC:DMPC or DHPC:POPC lipids were used. An increase in the rate of proton spin-lattice relaxation, T1-1, selectively detected for protons at different positions in the lipid molecule, showed that ibuprofen-SL is localized in the hydrophobic part of the lipid bilayer. As the concentration of ibuprofen-SL increases to 0.5 mol%, the distribution of positions of ibuprofen-SL across the bilayer becomes wider. In the presence of 20 mol% of cholesterol, ibuprofen-SL is displaced from the core of the membrane to a region closer to the head group of the bilayer. This displacement was also confirmed by the NMR NOESY experiment conducted with unlabeled ibuprofen. For bilayers containing unsaturated POPC lipids, the distribution of ibuprofen positions across the bilayer becomes narrower compared to the presence of saturated DMPC lipids.

KW - Ibuprofen

KW - Dimyristoylphosphatidylcholine

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Electrons

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85169015108&origin=inward&txGid=1706ef14f4b140940ba8cf2aa915b28d

U2 - 10.1016/j.bbamem.2023.184215

DO - 10.1016/j.bbamem.2023.184215

M3 - Article

C2 - 37633627

VL - 1865

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 8

M1 - 184215

ER -

ID: 55287210