TY - JOUR

T1 - Lipid-Mediated Effect of Glycyrrhizin on the Properties of the Transmembrane Domain of the E-Protein of the Sars-Cov-2 Virus

AU - Kononova, P. A.

AU - Selyutina, O. Yu

AU - Polyakov, N. E.

N1 - This study was carried out with the financial support of the Russian President’s Grant Council (grant MK-1580.2021.1.3).

PY - 2024/2

Y1 - 2024/2

N2 - The interaction of glycyrrhizin with the transmembrane domain of the E-protein of the SARS-CoV-2 virus (E-protein Trans-Membrane domain, ETM) in a homogeneous aqueous solution and in a model lipid membrane is studied using the selective nuclear Overhauser effect (selective NOESY) and NMR relaxation methods. The selective NOESY shows the presence of the interaction of glycyrrhizin with ETM in an aqueous solution, which is consistent with the published modeling data, which indicate the possibility of penetration of the glycyrrhizin molecule into the channel formed by the ETM molecules. However, this conclusion is not confirmed by the NOESY experiments in the model lipid membranes—bicelles. At the same time, the NMR relaxation method reveals the effect of glycyrrhizin on the mobility of both lipids and ETM molecules in bicelles. This suggests that GA affects the activity of the coronavirus E-protein indirectly through lipids.

AB - The interaction of glycyrrhizin with the transmembrane domain of the E-protein of the SARS-CoV-2 virus (E-protein Trans-Membrane domain, ETM) in a homogeneous aqueous solution and in a model lipid membrane is studied using the selective nuclear Overhauser effect (selective NOESY) and NMR relaxation methods. The selective NOESY shows the presence of the interaction of glycyrrhizin with ETM in an aqueous solution, which is consistent with the published modeling data, which indicate the possibility of penetration of the glycyrrhizin molecule into the channel formed by the ETM molecules. However, this conclusion is not confirmed by the NOESY experiments in the model lipid membranes—bicelles. At the same time, the NMR relaxation method reveals the effect of glycyrrhizin on the mobility of both lipids and ETM molecules in bicelles. This suggests that GA affects the activity of the coronavirus E-protein indirectly through lipids.

KW - NMR

KW - NOESY

KW - SARS-CoV-2 virus

KW - coronavirus E-protein

KW - glycyrrhizin

KW - lipid membranes

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85189538906&origin=inward&txGid=a5a0c05ad87bc7954d6641cafab7fb86

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001197827200015

UR - https://www.mendeley.com/catalogue/0555c3ad-95fc-30c9-a986-c3e08c68b9ed/

U2 - 10.1134/S1990793124010299

DO - 10.1134/S1990793124010299

M3 - Article

VL - 18

SP - 239

EP - 243

JO - Russian Journal of Physical Chemistry B

JF - Russian Journal of Physical Chemistry B

SN - 1990-7931

IS - 1

ER -