TY - JOUR

T1 - Lipid production by microalga Micractinium sp. IC-76 in a flat panel photobioreactor and its transesterification with cross-linked enzyme aggregates of Burkholderia cepacia lipase

AU - Piligaev, A. V.

AU - Sorokina, K. N.

AU - Samoylova, Y. V.

AU - Parmon, V. N.

N1 - Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/1/15

Y1 - 2018/1/15

N2 - In this study, the high-lipid biomass accumulation of the microalga Micractinium sp. IC-76 in a 110-L flat panel photobioreactor was studied in detail. The productivity of microalgae biomass during phototrophic cultivation was 35.6 ± 1.5 mg L−1 d−1. The maximum content of neutral lipids with a high saturated and monounsaturated fatty acid content (44.1%) was observed on the 17th day of cultivation, which was optimal for processing into biodiesel. The main fatty acids of lipids were C16:0, C16:2, and C18:2, with contents of 26.0, 15.6 and 29.8%, respectively. The enzymatic transesterification of Micractinium sp. IC-76 lipids using cross-linked enzyme aggregates (CLEAs) of Burkholderia cepacia lipase as biocatalysts was optimized by response surface methodology (RSM) to produce fatty acid methyl esters (FAMEs). This model determined that the optimum conditions for enzymatic transesterification of microalgal lipids with a maximal FAME yield of 92.3 ± 1.5% were a temperature of 38 °C, a methanol to oil molar ratio of 1:3.1, a CLEA of 9.1% and a water content of 2.5% relative to the oil weight. This model has a high correlation with the experimental data and can be used to model the production of FAMEs from the lipids of Micractinium sp. IC-76 during process scaling.

AB - In this study, the high-lipid biomass accumulation of the microalga Micractinium sp. IC-76 in a 110-L flat panel photobioreactor was studied in detail. The productivity of microalgae biomass during phototrophic cultivation was 35.6 ± 1.5 mg L−1 d−1. The maximum content of neutral lipids with a high saturated and monounsaturated fatty acid content (44.1%) was observed on the 17th day of cultivation, which was optimal for processing into biodiesel. The main fatty acids of lipids were C16:0, C16:2, and C18:2, with contents of 26.0, 15.6 and 29.8%, respectively. The enzymatic transesterification of Micractinium sp. IC-76 lipids using cross-linked enzyme aggregates (CLEAs) of Burkholderia cepacia lipase as biocatalysts was optimized by response surface methodology (RSM) to produce fatty acid methyl esters (FAMEs). This model determined that the optimum conditions for enzymatic transesterification of microalgal lipids with a maximal FAME yield of 92.3 ± 1.5% were a temperature of 38 °C, a methanol to oil molar ratio of 1:3.1, a CLEA of 9.1% and a water content of 2.5% relative to the oil weight. This model has a high correlation with the experimental data and can be used to model the production of FAMEs from the lipids of Micractinium sp. IC-76 during process scaling.

KW - Biodiesel

KW - Cross-linked enzyme aggregates

KW - Lipase

KW - Microalgae

KW - Response surface methodology

KW - Transesterification

KW - BIOFUEL PRODUCTION

KW - CONVERSION

KW - CHLORELLA-VULGARIS

KW - IMMOBILIZATION

KW - OIL

KW - BIODIESEL PRODUCTION

KW - BIOCATALYST

KW - ACCUMULATION

KW - SELECTION

KW - CULTIVATION

UR - http://www.scopus.com/inward/record.url?scp=85033607133&partnerID=8YFLogxK

U2 - 10.1016/j.enconman.2017.10.086

DO - 10.1016/j.enconman.2017.10.086

M3 - Article

AN - SCOPUS:85033607133

VL - 156

SP - 1

EP - 9

JO - Energy Conversion and Management

JF - Energy Conversion and Management

SN - 0196-8904

ER -