Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Lipid chain mobility and packing in DOPC bilayers at cryogenic temperatures. / Golysheva, Elena A.; Dzuba, Sergei A.
в: Chemistry and Physics of Lipids, Том 226, 104817, 01.01.2020.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Lipid chain mobility and packing in DOPC bilayers at cryogenic temperatures
AU - Golysheva, Elena A.
AU - Dzuba, Sergei A.
N1 - Copyright © 2019 Elsevier B.V. All rights reserved.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Low-temperature molecular mobility and packing in biological tissues are important for their survival upon cryopreservation. Electron paramagnetic resonance (EPR) in its pulsed version of electron spin echo (ESE) allows studying stochastic librations of spin-labeled molecules, the type of motion which dominates at low temperatures. These librations are characterized by the parameter <α2>τc where <α2> is the mean squared angular amplitude and τc is the correlation time for the motion. This parameter is known to be larger for higher temperature and for looser intermolecular structure. In this work, ESE data for the bilayers comprised of doubly-unsaturated DOPC (dioleoyl-glycero-phosphocholine) lipids and mono-unsaturated POPC (palmitoyl-oleoyl-glycero-phosphocholine) lipids with spin-labeled stearic acids added were obtained in the temperature range between 80 and 210 K; the results were compared also with the previously obtained data for fully-saturated DPPC (dipalmitoyl-glycero-phosphocholine) lipid bilayers [J. Phys. Chem. B 2014, 118, 12,478–12,485; Appl. Magn. Reson. 2018, 49, 1369–1383]. It turned out that for DOPC bilayers the <α2>τc values are of intermediate magnitude between those for POPC and DPPC bilayers, which implies an intermediate density of lipid packing. A possible explanation of this result could be rearrangement at cryogenic temperatures of the DOPC lipid tails, with their terminal segments folding cooperatively. This interpretation is also in agreement with the known thermodynamic properties of gel-fluid transition for DOPC bilayer.
AB - Low-temperature molecular mobility and packing in biological tissues are important for their survival upon cryopreservation. Electron paramagnetic resonance (EPR) in its pulsed version of electron spin echo (ESE) allows studying stochastic librations of spin-labeled molecules, the type of motion which dominates at low temperatures. These librations are characterized by the parameter <α2>τc where <α2> is the mean squared angular amplitude and τc is the correlation time for the motion. This parameter is known to be larger for higher temperature and for looser intermolecular structure. In this work, ESE data for the bilayers comprised of doubly-unsaturated DOPC (dioleoyl-glycero-phosphocholine) lipids and mono-unsaturated POPC (palmitoyl-oleoyl-glycero-phosphocholine) lipids with spin-labeled stearic acids added were obtained in the temperature range between 80 and 210 K; the results were compared also with the previously obtained data for fully-saturated DPPC (dipalmitoyl-glycero-phosphocholine) lipid bilayers [J. Phys. Chem. B 2014, 118, 12,478–12,485; Appl. Magn. Reson. 2018, 49, 1369–1383]. It turned out that for DOPC bilayers the <α2>τc values are of intermediate magnitude between those for POPC and DPPC bilayers, which implies an intermediate density of lipid packing. A possible explanation of this result could be rearrangement at cryogenic temperatures of the DOPC lipid tails, with their terminal segments folding cooperatively. This interpretation is also in agreement with the known thermodynamic properties of gel-fluid transition for DOPC bilayer.
KW - Biological membranes
KW - Cryopreservation
KW - Electron spin echo
KW - EPR
KW - Gel-fluid transition
KW - Spin labels
KW - Stearic acid
KW - CHOLESTEROL
KW - ECHO
KW - MEMBRANES
KW - MODEL
KW - DYNAMICAL TRANSITION
KW - GLASS-TRANSITION
KW - ELECTRON-PARAMAGNETIC-RESONANCE
KW - ORDER PARAMETERS
KW - SPIN-LABELED LIPIDS
KW - IONIC LIQUIDS
UR - http://www.scopus.com/inward/record.url?scp=85074437477&partnerID=8YFLogxK
U2 - 10.1016/j.chemphyslip.2019.104817
DO - 10.1016/j.chemphyslip.2019.104817
M3 - Article
C2 - 31525380
AN - SCOPUS:85074437477
VL - 226
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
SN - 0009-3084
M1 - 104817
ER -
ID: 22077993