TY - JOUR

T1 - Knockout of the Tnfa Gene Decreases Influenza Virus-Induced Histological Reactions in Laboratory Mice

AU - Savenkova, Darya A

AU - Gudymo, Andrey S

AU - Korablev, Alexey N

AU - Taranov, Oleg S

AU - Bazovkina, Darya V

AU - Danilchenko, Nataliya V

AU - Perfilyeva, Olga N

AU - Ivleva, Elena K

AU - Moiseeva, Anastasiya A

AU - Bulanovich, Yulia A

AU - Roshchina, Elena V

AU - Serova, Irina A

AU - Battulin, Nariman R

AU - Kulikova, Elizabeth A

AU - Yudkin, Dmitry V

N1 - State Assignment No. 10/21 (FBRI SRC VB ‘Vector’ Rospotrebnadzor) supported this research. Публикация для корректировки.

PY - 2024/1

Y1 - 2024/1

N2 - Tumor necrosis factor alpha (TNF-α) is a cytokine that is responsible for many processes associated with immune response and inflammation. It is involved in the development of an antiviral response to many virus infections. This factor was shown to be activated in influenza A virus infection, which enhances production of other cytokines. The overexpression of these cytokines can lead to a cytokine storm. To study the role of TNF-α in the development of pathologies associated with viral infection, we generated a Tnfa knockout mouse strain. We demonstrated that these mice were characterized by a significant increase in the number of viral genomes compared to that in the parental strain, but the amount of live virus did not differ. A histopathology of the lungs in the genetically modified animals was significantly lower in terms of interalveolar septal infiltration. The generated model may be used to further study pathological processes in viral infections.

AB - Tumor necrosis factor alpha (TNF-α) is a cytokine that is responsible for many processes associated with immune response and inflammation. It is involved in the development of an antiviral response to many virus infections. This factor was shown to be activated in influenza A virus infection, which enhances production of other cytokines. The overexpression of these cytokines can lead to a cytokine storm. To study the role of TNF-α in the development of pathologies associated with viral infection, we generated a Tnfa knockout mouse strain. We demonstrated that these mice were characterized by a significant increase in the number of viral genomes compared to that in the parental strain, but the amount of live virus did not differ. A histopathology of the lungs in the genetically modified animals was significantly lower in terms of interalveolar septal infiltration. The generated model may be used to further study pathological processes in viral infections.

KW - Animals

KW - Mice

KW - Cytokines/genetics

KW - Influenza A virus

KW - Mice, Knockout

KW - Tumor Necrosis Factor-alpha/genetics

KW - Orthomyxoviridae Infections/pathology

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85183271107&origin=inward&txGid=cd42a6059fed10d99870d87977a6e467

U2 - 10.3390/ijms25021156

DO - 10.3390/ijms25021156

M3 - Article

C2 - 38256229

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 2

M1 - 1156

ER -