Ketamine Reverses Depressive-Like Behavior Induced by Optogenetic Stimulation of Glutamatergic Neurons in the Dorsal Hippocampus

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Ketamine Reverses Depressive-Like Behavior Induced by Optogenetic Stimulation of Glutamatergic Neurons in the Dorsal Hippocampus. / Drozd, U. S.; Sukhareva, E. V.; Bulygina, V. V. и др.

в: Neurochemical Journal, Том 19, № 3, 10, 09.2025, стр. 366-373.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{43c39a885965492cad4a5840b660d97b,

title = "Ketamine Reverses Depressive-Like Behavior Induced by Optogenetic Stimulation of Glutamatergic Neurons in the Dorsal Hippocampus",

abstract = "The hippocampus is one of the brain structures whose functions and morphology are impaired in depression. The fast-acting antidepressant ketamine reverses these impairments, but the mechanisms of its action are still not fully understood. Optogenetic stimulation of glutamatergic neurons in the CA1 region of the dorsal hippocampus of rats preliminary injected with vectors expressing photosensitive channelrhodopsin led to the manifestation of a sign of depressive-like behavior—an increase in the time of immobility in the tail suspension test, compared to control animals. Immunohistochemical analysis of the expression of the early response protein c-Fos in the CA1 region of the hippocampus confirmed the activation of pyramidal neurons under the influence of light, revealing their involvement in the development of depressive-like behavior. Administration of a subanesthetic dose of ketamine prevented the manifestation of depressive-like behavior trait induced by optogenetic activation of glutamatergic neurons in the CA1 region of the dorsal hippocampus and abolished the increase in c-fos mRNA levels induced by optical stimulation. Thus, we demonstrated for the first time the ability of ketamine to reverse depressive-like behavior induced by optogenetic stimulation of the activity of dorsal hippocampal glutamatergic neurons. Overall, the results indicate the important role of dorsal hippocampal glutamatergic neurons in the regulation of psychoemotional behavioral responses and their sensitivity to ketamine administration.",

keywords = "dorsal hippocampus, glutamatergic neurons, CA1 region, optogenetics, channelrhodopsin, ketamine, depressive-like behavior, tail suspension test, c-Fos protein",

author = "Drozd, {U. S.} and Sukhareva, {E. V.} and Bulygina, {V. V.} and Kalinina, {T. S.} and Dygalo, {N. N.} and Lanshakov, {D. A.}",

note = "Ketamine Reverses Depressive-Like Behavior Induced by Optogenetic Stimulation of Glutamatergic Neurons in the Dorsal Hippocampus / U. S. Drozd, E. V. Sukhareva, V. V. Bulygina [et al.] // Neurochemical Journal. – 2025. – Vol. 19, No. 3. – P. 366-373. – DOI 10.1134/S1819712425700412. – EDN KTCUWJ. The work was supported by the budget project FWNR-2022-0023.",

year = "2025",

month = sep,

doi = "10.1134/s1819712425700412",

language = "English",

volume = "19",

pages = "366--373",

journal = "Neurochemical Journal",

issn = "1819-7124",

publisher = "Общество с ограниченной ответственностью Международная академическая издательская компания {"}Наука/Интерпериодика{"}",

number = "3",

}

RIS

TY - JOUR

T1 - Ketamine Reverses Depressive-Like Behavior Induced by Optogenetic Stimulation of Glutamatergic Neurons in the Dorsal Hippocampus

AU - Drozd, U. S.

AU - Sukhareva, E. V.

AU - Bulygina, V. V.

AU - Kalinina, T. S.

AU - Dygalo, N. N.

AU - Lanshakov, D. A.

N1 - Ketamine Reverses Depressive-Like Behavior Induced by Optogenetic Stimulation of Glutamatergic Neurons in the Dorsal Hippocampus / U. S. Drozd, E. V. Sukhareva, V. V. Bulygina [et al.] // Neurochemical Journal. – 2025. – Vol. 19, No. 3. – P. 366-373. – DOI 10.1134/S1819712425700412. – EDN KTCUWJ. The work was supported by the budget project FWNR-2022-0023.

PY - 2025/9

Y1 - 2025/9

N2 - The hippocampus is one of the brain structures whose functions and morphology are impaired in depression. The fast-acting antidepressant ketamine reverses these impairments, but the mechanisms of its action are still not fully understood. Optogenetic stimulation of glutamatergic neurons in the CA1 region of the dorsal hippocampus of rats preliminary injected with vectors expressing photosensitive channelrhodopsin led to the manifestation of a sign of depressive-like behavior—an increase in the time of immobility in the tail suspension test, compared to control animals. Immunohistochemical analysis of the expression of the early response protein c-Fos in the CA1 region of the hippocampus confirmed the activation of pyramidal neurons under the influence of light, revealing their involvement in the development of depressive-like behavior. Administration of a subanesthetic dose of ketamine prevented the manifestation of depressive-like behavior trait induced by optogenetic activation of glutamatergic neurons in the CA1 region of the dorsal hippocampus and abolished the increase in c-fos mRNA levels induced by optical stimulation. Thus, we demonstrated for the first time the ability of ketamine to reverse depressive-like behavior induced by optogenetic stimulation of the activity of dorsal hippocampal glutamatergic neurons. Overall, the results indicate the important role of dorsal hippocampal glutamatergic neurons in the regulation of psychoemotional behavioral responses and their sensitivity to ketamine administration.

AB - The hippocampus is one of the brain structures whose functions and morphology are impaired in depression. The fast-acting antidepressant ketamine reverses these impairments, but the mechanisms of its action are still not fully understood. Optogenetic stimulation of glutamatergic neurons in the CA1 region of the dorsal hippocampus of rats preliminary injected with vectors expressing photosensitive channelrhodopsin led to the manifestation of a sign of depressive-like behavior—an increase in the time of immobility in the tail suspension test, compared to control animals. Immunohistochemical analysis of the expression of the early response protein c-Fos in the CA1 region of the hippocampus confirmed the activation of pyramidal neurons under the influence of light, revealing their involvement in the development of depressive-like behavior. Administration of a subanesthetic dose of ketamine prevented the manifestation of depressive-like behavior trait induced by optogenetic activation of glutamatergic neurons in the CA1 region of the dorsal hippocampus and abolished the increase in c-fos mRNA levels induced by optical stimulation. Thus, we demonstrated for the first time the ability of ketamine to reverse depressive-like behavior induced by optogenetic stimulation of the activity of dorsal hippocampal glutamatergic neurons. Overall, the results indicate the important role of dorsal hippocampal glutamatergic neurons in the regulation of psychoemotional behavioral responses and their sensitivity to ketamine administration.

KW - dorsal hippocampus

KW - glutamatergic neurons

KW - CA1 region

KW - optogenetics

KW - channelrhodopsin

KW - ketamine

KW - depressive-like behavior

KW - tail suspension test

KW - c-Fos protein

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001615561700019

UR - https://elibrary.ru/item.asp?id=83185742

UR - https://www.mendeley.com/catalogue/b7d9cf60-1c1b-359c-9b31-1a4336883af3/

U2 - 10.1134/s1819712425700412

DO - 10.1134/s1819712425700412

M3 - Article

VL - 19

SP - 366

EP - 373

JO - Neurochemical Journal

JF - Neurochemical Journal

SN - 1819-7124

IS - 3

M1 - 10

ER -

ID: 80857015