TY - JOUR

T1 - Ketamine improves depressive symptoms caused by stress and induces c-Fos expression in the brain resembling some effects of stress

AU - Dygalo, N.

AU - Drozd, U. S.

AU - Kalinina, T. S.

AU - Sukhareva, E. V.

AU - Bulygina, V. V.

AU - Shishkina, G. T.

AU - Lanshakov, D. A.

PY - 2019

Y1 - 2019

N2 - Purpose: Optogenetic stimulation of the hippocampal pyramidal glutamatergic neurons resulted in a depressive-like behavior [1] which was accompanied by activation of neurons in the hippocampal CA1 region and prefrontal cortex [2]. In the present study, using expression of c-Fos, we examined which changes in neuronal activity could be associated with antidepressant effects of ketamine, an antagonist of glutamate NMDA receptors. Methods: Adult male Wistar rats were injected with ketamine (15 mg/kg, i.p.) or saline. Two hours later, half of the animals of each treatment group were subjected to the tail suspension test for 6 minutes (stress) and their behavioral responses were evaluated. One hour after the test, brains and samples of the brain tissue of these animals and unexposed to stress rats of both groups were harvested for immunofluorescence analysis of c-Fos, Satb2 and Calretinin proteins and measurement of c-fos mRNA levels by RT real-time PCR. Plasma corticosterone levels were determined by ELISA. Statistical analysis was made using one- and two-way ANOVA. Results: Stress of the tail suspension test significantly (p

AB - Purpose: Optogenetic stimulation of the hippocampal pyramidal glutamatergic neurons resulted in a depressive-like behavior [1] which was accompanied by activation of neurons in the hippocampal CA1 region and prefrontal cortex [2]. In the present study, using expression of c-Fos, we examined which changes in neuronal activity could be associated with antidepressant effects of ketamine, an antagonist of glutamate NMDA receptors. Methods: Adult male Wistar rats were injected with ketamine (15 mg/kg, i.p.) or saline. Two hours later, half of the animals of each treatment group were subjected to the tail suspension test for 6 minutes (stress) and their behavioral responses were evaluated. One hour after the test, brains and samples of the brain tissue of these animals and unexposed to stress rats of both groups were harvested for immunofluorescence analysis of c-Fos, Satb2 and Calretinin proteins and measurement of c-fos mRNA levels by RT real-time PCR. Plasma corticosterone levels were determined by ELISA. Statistical analysis was made using one- and two-way ANOVA. Results: Stress of the tail suspension test significantly (p

KW - ACTIVATION

KW - CA1

UR - https://www.mendeley.com/catalogue/a17e796b-840f-376e-a6d4-55fa91677a03/

U2 - 10.1016/j.euroneuro.2018.11.348

DO - 10.1016/j.euroneuro.2018.11.348

M3 - Meeting Abstract

VL - 29

SP - S212-S212

JO - European Neuropsychopharmacology

JF - European Neuropsychopharmacology

SN - 0924-977X

T2 - 31st Congress of the European-College-of-Neuropsychopharmacology (ECNP)

Y2 - 6 October 2018 through 9 October 2018

ER -