TY - JOUR

T1 - Isolation and characterization of a Novel Klebsiella pneumoniae N4-like bacteriophage KP8

AU - Morozova, Vera

AU - Babkin, Igor

AU - Kozlova, Yuliya

AU - Baykov, Ivan

AU - Bokovaya, Olga

AU - Tikunov, Artem

AU - Ushakova, Tatyana

AU - Bardasheva, Alevtina

AU - Ryabchikova, Elena

AU - Zelentsova, Ekaterina

AU - Tikunova, Nina

PY - 2019/12/2

Y1 - 2019/12/2

N2 - Klebsiella pneumoniae is a common pathogen, associated with a wide spectrum of infections, and clinical isolates of K. pneumoniae often possess multiple antibiotic resistances. Here, we describe a novel lytic N4-like bacteriophage KP8, specific to K. pneumoniae, including its genome, partial structural proteome, biological properties, and proposed taxonomy. Electron microscopy revealed that KP8 belongs to the Podoviridae family. The size of the KP8 genome was 73,679 bp, and it comprised 97 putative open reading frames. Comparative genome analysis revealed that the KP8 genome possessed the highest similarity to the genomes of Enquatrovirus and Gamaleyavirus phages, which are N4-like podoviruses. In addition, the KP8 genome showed gene synteny typical of the N4-like podoviruses and contained the gene encoding a large virion-encapsulated RNA polymerase. Phylogenetic analysis of the KP8 genome revealed that the KP8 genome formed a distinct branch within the clade, which included the members of Enquatrovirus and Gamaleyavirus genera besides KP8. The average evolutionary divergences KP8/Enquatrovirus and KP8/Gamaleyavirus were 0.466 and 0.447 substitutions per site (substitutes/site), respectively, similar to that between Enquatrovirus and Gamaleyavirus genera (0.468 substitutes/site). The obtained data suggested that Klebsiella phage KP8 differs from other similar phages and may represent a new genus within the N4-like phages.

AB - Klebsiella pneumoniae is a common pathogen, associated with a wide spectrum of infections, and clinical isolates of K. pneumoniae often possess multiple antibiotic resistances. Here, we describe a novel lytic N4-like bacteriophage KP8, specific to K. pneumoniae, including its genome, partial structural proteome, biological properties, and proposed taxonomy. Electron microscopy revealed that KP8 belongs to the Podoviridae family. The size of the KP8 genome was 73,679 bp, and it comprised 97 putative open reading frames. Comparative genome analysis revealed that the KP8 genome possessed the highest similarity to the genomes of Enquatrovirus and Gamaleyavirus phages, which are N4-like podoviruses. In addition, the KP8 genome showed gene synteny typical of the N4-like podoviruses and contained the gene encoding a large virion-encapsulated RNA polymerase. Phylogenetic analysis of the KP8 genome revealed that the KP8 genome formed a distinct branch within the clade, which included the members of Enquatrovirus and Gamaleyavirus genera besides KP8. The average evolutionary divergences KP8/Enquatrovirus and KP8/Gamaleyavirus were 0.466 and 0.447 substitutions per site (substitutes/site), respectively, similar to that between Enquatrovirus and Gamaleyavirus genera (0.468 substitutes/site). The obtained data suggested that Klebsiella phage KP8 differs from other similar phages and may represent a new genus within the N4-like phages.

KW - Bacteriophage

KW - Enquatrovirus

KW - Gamaleyavirus

KW - Klebsiella pneumoniae

KW - N4-like podovirus

KW - COMPLETE GENOME SEQUENCE

KW - PHAGES

KW - GENUS

KW - IDENTIFICATION

KW - bacteriophage

UR - http://www.scopus.com/inward/record.url?scp=85076274541&partnerID=8YFLogxK

U2 - 10.3390/v11121115

DO - 10.3390/v11121115

M3 - Article

C2 - 31810319

AN - SCOPUS:85076274541

VL - 11

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 12

M1 - 1115

ER -