TY - JOUR

T1 - Irreversible lipid phase transition detected in a porcine oocyte at chilling

AU - Okotrub, K A

AU - Omelchenko, A N

AU - Chuyko, E A

AU - Amstislavskyu, S Y

AU - Surovtsev, N V

N1 - This work was supported by the budget projects of the ICG SB RAS No. FWNR-2022-0005, FWNR-2022-0023 and by the Ministry of Science and Higher Education of the Russian Federation (No 1023032300239–8). The studies were implemented using the equipment of the Center for Genetic Resources of Laboratory Animals at ICG SB RAS (RFMEFI62119X0023). Raman experiments were performed in the Multiple-access center “High resolution spectroscopy of gases and condensed matters” in IA&E SB RAS (Novosibirsk, Russia).

PY - 2024/3

Y1 - 2024/3

N2 - Under physiological conditions, the membranes and lipid droplets of germ cells are in a conformationally disordered phase. Typically, during cooling, lipids undergo the transition to ordered phases and, upon heating, melt into a disordered phase. In this communication, we report the lipid phase transition in lipid droplets observed in porcine oocytes. Upon cooling, a sharp lipid phase transition from conformationally disordered to ordered state was detected within the temperature range between 20 and 15 °C. Subsequent heating to 45 °C does not return lipids to their original phase state. To the best of our knowledge, this is the first observation of an irreversible phase transition in lipid droplets of biological cells with native lipid composition.

AB - Under physiological conditions, the membranes and lipid droplets of germ cells are in a conformationally disordered phase. Typically, during cooling, lipids undergo the transition to ordered phases and, upon heating, melt into a disordered phase. In this communication, we report the lipid phase transition in lipid droplets observed in porcine oocytes. Upon cooling, a sharp lipid phase transition from conformationally disordered to ordered state was detected within the temperature range between 20 and 15 °C. Subsequent heating to 45 °C does not return lipids to their original phase state. To the best of our knowledge, this is the first observation of an irreversible phase transition in lipid droplets of biological cells with native lipid composition.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85183484169&origin=inward&txGid=9b350c1295f251a71feafe87b537fe03

U2 - 10.1016/j.cryobiol.2024.104850

DO - 10.1016/j.cryobiol.2024.104850

M3 - Article

C2 - 38242275

VL - 114

JO - Cryobiology

JF - Cryobiology

SN - 0011-2240

M1 - 104850

ER -