TY - JOUR

T1 - Irreversible lipid phase transition detected in a porcine oocyte at chilling

AU - Okotrub, K A

AU - Omelchenko, A N

AU - Chuyko, E A

AU - Amstislavskyu, S Y

AU - Surovtsev, N V

N1 - Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.

PY - 2024/3

Y1 - 2024/3

N2 - Under physiological conditions, the membranes and lipid droplets of germ cells are in a conformationally disordered phase. Typically, during cooling, lipids undergo the transition to ordered phases and, upon heating, melt into a disordered phase. In this communication, we report the lipid phase transition in lipid droplets observed in porcine oocytes. Upon cooling, a sharp lipid phase transition from conformationally disordered to ordered state was detected within the temperature range between 20 and 15 °C. Subsequent heating to 45 °C does not return lipids to their original phase state. To the best of our knowledge, this is the first observation of an irreversible phase transition in lipid droplets of biological cells with native lipid composition.

AB - Under physiological conditions, the membranes and lipid droplets of germ cells are in a conformationally disordered phase. Typically, during cooling, lipids undergo the transition to ordered phases and, upon heating, melt into a disordered phase. In this communication, we report the lipid phase transition in lipid droplets observed in porcine oocytes. Upon cooling, a sharp lipid phase transition from conformationally disordered to ordered state was detected within the temperature range between 20 and 15 °C. Subsequent heating to 45 °C does not return lipids to their original phase state. To the best of our knowledge, this is the first observation of an irreversible phase transition in lipid droplets of biological cells with native lipid composition.

U2 - 10.1016/j.cryobiol.2024.104850

DO - 10.1016/j.cryobiol.2024.104850

M3 - Article

C2 - 38242275

VL - 114

JO - Cryobiology

JF - Cryobiology

SN - 0011-2240

M1 - 104850

ER -