TY - JOUR

T1 - Involvement of Abscisic Acid in Transition of Pea (Pisum sativum L.) Seeds from Germination to Post-Germination Stages

AU - Smolikova, Galina

AU - Krylova, Ekaterina

AU - Petřík, Ivan

AU - Vilis, Polina

AU - Vikhorev, Aleksander

AU - Strygina, Ksenia

AU - Strnad, Miroslav

AU - Frolov, Andrej

AU - Khlestkina, Elena

AU - Medvedev, Sergei

N1 - This research was funded by the Russian Science Foundation, grant no. 20-16-00086 (50% of the study dealing with annotation of ABA-associated DEGs and DNA methylation in the promoters of the ABA-depended genes), the grant IGA from Palacky University no. IGA PrF_2023_031 (25% of the study dealing with quantitation of ABA and ABA-related metabolites) and the K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, theme no. 1021052706080-4-1.6.11 (25% of the study dealing with the bioinformatics and preparing the plant material). Публикация для корректировки.

PY - 2024/1/11

Y1 - 2024/1/11

N2 - The transition from seed to seedling represents a critical developmental step in the life cycle of higher plants, dramatically affecting plant ontogenesis and stress tolerance. The release from dormancy to acquiring germination ability is defined by a balance of phytohormones, with the substantial contribution of abscisic acid (ABA), which inhibits germination. We studied the embryonic axis of Pisum sativum L. before and after radicle protrusion. Our previous work compared RNA sequencing-based transcriptomics in the embryonic axis isolated before and after radicle protrusion. The current study aims to analyze ABA-dependent gene regulation during the transition of the embryonic axis from the germination to post-germination stages. First, we determined the levels of abscisates (ABA, phaseic acid, dihydrophaseic acid, and neo-phaseic acid) using ultra-high-performance liquid chromatography–tandem mass spectrometry. Second, we made a detailed annotation of ABA-associated genes using RNA sequencing-based transcriptome profiling. Finally, we analyzed the DNA methylation patterns in the promoters of the PsABI3, PsABI4, and PsABI5 genes. We showed that changes in the abscisate profile are characterized by the accumulation of ABA catabolites, and the ABA-related gene profile is accompanied by the upregulation of genes controlling seedling development and the downregulation of genes controlling water deprivation. The expression of ABI3, ABI4, and ABI5, which encode crucial transcription factors during late maturation, was downregulated by more than 20-fold, and their promoters exhibited high levels of methylation already at the late germination stage. Thus, although ABA remains important, other regulators seems to be involved in the transition from seed to seedling.

AB - The transition from seed to seedling represents a critical developmental step in the life cycle of higher plants, dramatically affecting plant ontogenesis and stress tolerance. The release from dormancy to acquiring germination ability is defined by a balance of phytohormones, with the substantial contribution of abscisic acid (ABA), which inhibits germination. We studied the embryonic axis of Pisum sativum L. before and after radicle protrusion. Our previous work compared RNA sequencing-based transcriptomics in the embryonic axis isolated before and after radicle protrusion. The current study aims to analyze ABA-dependent gene regulation during the transition of the embryonic axis from the germination to post-germination stages. First, we determined the levels of abscisates (ABA, phaseic acid, dihydrophaseic acid, and neo-phaseic acid) using ultra-high-performance liquid chromatography–tandem mass spectrometry. Second, we made a detailed annotation of ABA-associated genes using RNA sequencing-based transcriptome profiling. Finally, we analyzed the DNA methylation patterns in the promoters of the PsABI3, PsABI4, and PsABI5 genes. We showed that changes in the abscisate profile are characterized by the accumulation of ABA catabolites, and the ABA-related gene profile is accompanied by the upregulation of genes controlling seedling development and the downregulation of genes controlling water deprivation. The expression of ABI3, ABI4, and ABI5, which encode crucial transcription factors during late maturation, was downregulated by more than 20-fold, and their promoters exhibited high levels of methylation already at the late germination stage. Thus, although ABA remains important, other regulators seems to be involved in the transition from seed to seedling.

KW - ABA-associated genes

KW - DNA methylation

KW - Pisum sativumL

KW - abscisic acid

KW - embryonic axis

KW - seed-to-seedling transition

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85183192752&origin=inward&txGid=9295d209560428c2a52858aad3940136

UR - https://www.mendeley.com/catalogue/3a21eff3-6d59-37de-b05e-0d6547cba726/

U2 - 10.3390/plants13020206

DO - 10.3390/plants13020206

M3 - Article

C2 - 38256760

VL - 13

JO - Plants

JF - Plants

SN - 2223-7747

IS - 2

M1 - 206

ER -