TY - JOUR

T1 - Intrachromosomal rearrangements in rodents from the perspective of comparative region-specific painting

AU - Romanenko, Svetlana A.

AU - Serdyukova, Natalya A.

AU - Perelman, Polina L.

AU - Pavlova, Svetlana V.

AU - Bulatova, Nina S.

AU - Golenishchev, Feodor N.

AU - Stanyon, Roscoe

AU - Graphodatsky, Alexander S.

PY - 2017/8/30

Y1 - 2017/8/30

N2 - It has long been hypothesized that chromosomal rearrangements play a central role in different evolutionary processes, particularly in speciation and adaptation. Interchromosomal rearrangements have been extensively mapped using chromosome painting. However, intrachromosomal rearrangements have only been described using molecular cytogenetics in a limited number of mammals, including a few rodent species. This situation is unfortunate because intrachromosomal rearrangements are more abundant than interchromosomal rearrangements and probably contain essential phylogenomic information. Significant progress in the detection of intrachromosomal rearrangement is now possible, due to recent advances in molecular biology and bioinformatics. We investigated the level of intrachromosomal rearrangement in the Arvicolinae subfamily, a species-rich taxon characterized by very high rate of karyotype evolution. We made a set of region specific probes by microdissection for a single syntenic region represented by the p-arm of chromosome 1 of Alexandromys oeconomus, and hybridized the probes onto the chromosomes of four arvicolines (Microtus agrestis, Microtus arvalis, Myodes rutilus, andDicrostonyx torquatus). These experiments allowed us to show the intrachromosomal rearrangements in the subfamily at a significantly higher level of resolution than previously described. We found a number of paracentric inversions in the karyotypes of M. agrestis and M. rutilus, as well as multiple inversions and a centromere shift in the karyotype of M. arvalis. We propose that during karyotype evolution, arvicolines underwent a significant number of complex intrachromosomal rearrangements that were not previously detected.

AB - It has long been hypothesized that chromosomal rearrangements play a central role in different evolutionary processes, particularly in speciation and adaptation. Interchromosomal rearrangements have been extensively mapped using chromosome painting. However, intrachromosomal rearrangements have only been described using molecular cytogenetics in a limited number of mammals, including a few rodent species. This situation is unfortunate because intrachromosomal rearrangements are more abundant than interchromosomal rearrangements and probably contain essential phylogenomic information. Significant progress in the detection of intrachromosomal rearrangement is now possible, due to recent advances in molecular biology and bioinformatics. We investigated the level of intrachromosomal rearrangement in the Arvicolinae subfamily, a species-rich taxon characterized by very high rate of karyotype evolution. We made a set of region specific probes by microdissection for a single syntenic region represented by the p-arm of chromosome 1 of Alexandromys oeconomus, and hybridized the probes onto the chromosomes of four arvicolines (Microtus agrestis, Microtus arvalis, Myodes rutilus, andDicrostonyx torquatus). These experiments allowed us to show the intrachromosomal rearrangements in the subfamily at a significantly higher level of resolution than previously described. We found a number of paracentric inversions in the karyotypes of M. agrestis and M. rutilus, as well as multiple inversions and a centromere shift in the karyotype of M. arvalis. We propose that during karyotype evolution, arvicolines underwent a significant number of complex intrachromosomal rearrangements that were not previously detected.

KW - Centromere shift

KW - Chromosome painting

KW - Comparative cytogenetics

KW - Fluorescence in situ hybridization

KW - Inversion

KW - Microdissection

KW - Small mammals

KW - Voles

KW - MOLECULAR CYTOGENETIC ANALYSIS

KW - X-CHROMOSOME

KW - IN-SITU HYBRIDIZATION

KW - comparative cytogenetics

KW - inversion

KW - SPECIATION

KW - MAMMALIAN CHROMOSOME

KW - KARYOTYPE EVOLUTION

KW - FIELD VOLE

KW - GENOME HOMOLOGY

KW - fluorescence in situ hybridization

KW - chromosome painting

KW - voles

KW - microdissection

KW - ARVICOLINAE CRICETIDAE

KW - centromere shift

KW - small mammals

KW - GENUS MICROTUS

UR - http://www.scopus.com/inward/record.url?scp=85028652239&partnerID=8YFLogxK

U2 - 10.3390/genes8090215

DO - 10.3390/genes8090215

M3 - Article

C2 - 28867774

AN - SCOPUS:85028652239

VL - 8

JO - Genes

JF - Genes

SN - 2073-4425

IS - 9

M1 - 215

ER -