TY - JOUR

T1 - Insulator Protein CP190 Regulates Expression оf Spermatocyte Differentiation Genes in Drosophila melanogaster Male Germline

AU - Romanov, S. E.

AU - Shloma, V. V.

AU - Koryakov, D. E.

AU - Belyakin, S. N.

AU - Laktionov, P. P.

N1 - Funding Information: The reported study was funded by Russian Foundation for Basic Research, project nos. 19-34-90108, 17-00-00181, and 19-04-00872, and supported by Basic Science Research Program FWGZ-2021-0017 (122011900429-5). Publisher Copyright: © 2023, Pleiades Publishing, Inc.

PY - 2023/2

Y1 - 2023/2

N2 - CP190 protein is one of the key components of Drosophila insulator complexes, and its study is important for understanding the mechanisms of gene regulation during cell differentiation. However, Cp190 mutants die before reaching adulthood, which significantly complicates the study of its functions in imago. To overcome this problem and to investigate the regulatory effects of CP190 in adult tissues development, we have designed a conditional rescue system for Cp190 mutants. Using Cre/loxP-mediated recombination, the rescue construct containing Cp190 coding sequence is effectively eliminated specifically in spermatocytes, allowing us to study the effect of the mutation in male germ cells. Using high-throughput transcriptome analysis we determined the function of CP190 on gene expression in germline cells. Cp190 mutation was found to have opposite effects on tissue-specific genes, which expression is repressed by CP190, and housekeeping genes, that require CP190 for activation. Mutation of Cp190 also promoted expression of a set of spermatocyte differentiation genes that are regulated by tMAC transcriptional complex. Our results indicate that the main function of CP190 in the process of spermatogenesis is the coordination of interactions between differentiation genes and their specific transcriptional activators.

AB - CP190 protein is one of the key components of Drosophila insulator complexes, and its study is important for understanding the mechanisms of gene regulation during cell differentiation. However, Cp190 mutants die before reaching adulthood, which significantly complicates the study of its functions in imago. To overcome this problem and to investigate the regulatory effects of CP190 in adult tissues development, we have designed a conditional rescue system for Cp190 mutants. Using Cre/loxP-mediated recombination, the rescue construct containing Cp190 coding sequence is effectively eliminated specifically in spermatocytes, allowing us to study the effect of the mutation in male germ cells. Using high-throughput transcriptome analysis we determined the function of CP190 on gene expression in germline cells. Cp190 mutation was found to have opposite effects on tissue-specific genes, which expression is repressed by CP190, and housekeeping genes, that require CP190 for activation. Mutation of Cp190 also promoted expression of a set of spermatocyte differentiation genes that are regulated by tMAC transcriptional complex. Our results indicate that the main function of CP190 in the process of spermatogenesis is the coordination of interactions between differentiation genes and their specific transcriptional activators.

KW - CP190

KW - Drosophila melanogaster

KW - insulator proteins

KW - RNA-seq

UR - http://www.scopus.com/inward/record.url?scp=85141165481&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/9172bdca-d41e-3cd2-9cc6-cdf57ba47bc0/

U2 - 10.1134/S0026893323010120

DO - 10.1134/S0026893323010120

M3 - Article

AN - SCOPUS:85141165481

VL - 57

SP - 113

EP - 126

JO - Molecular Biology

JF - Molecular Biology

SN - 0026-8933

IS - 1

ER -