TY - GEN

T1 - In vitro BNCT mediated boronophenylalanine evaluation on melanoma and glioma cells

AU - Каныгин, Владимир Владимирович

AU - Касатова, Анна Исмагиловна

AU - Кичигин, Александр Иванович

AU - Завьялов, Евгений Леонидович

AU - Разумов, Иван Алексеевич

AU - Таскаев, Сергей Юрьевич

N1 - In vitro BNCT mediated boronophenylalanine evaluation on melanoma and glioma cells / V. Kanygin, A. Kasatova, A. Kichigin [et al.] // 19th International Congress on Neutron Capture Therapy (ICNCT 19) : Book of Abstracts, Granada, Spain, 27 сентября – 01 2021 года. – Granada, Spain: International Atomic Energy Agency, 2021. – P. 179.

PY - 2021

Y1 - 2021

N2 - To identify the dependence of the efficiency of boron neutron capture therapy on glioma U251 and melanoma SK-Mel28 cell lines neutron irradiation in presence of 10B at the accelerator based epithermal neutron source at Budker Institute of Nuclear Physics was conducted. Cells were incubated with different concentrations of 10B labeled boronophenylalanine to the growth medium for 24 hours. The samples were placed in polymethyl methacrylate phantom on a depth of 36 mm under the neutron-generating target with following irradiation of the cells by neutron flux. Colony forming assay was performed to evaluate clonogenic capabilities of irradiated glioma and melanoma cells. I t showed that 10B increased by irradiation with neutron beam, leads to decrease in cell survival. It showed that the cells surviving decreased with increasing of 10B after irradiation with neutron beam. Concentrations of 10B in U251 and SK-Mel28 cells were detected with atomic emission spectrometer ICPE-9820 (Shimadzu, Japan) that of confirm the dependence of boron accumulation and cell death. The report presents and discusses the results of this investigation.

AB - To identify the dependence of the efficiency of boron neutron capture therapy on glioma U251 and melanoma SK-Mel28 cell lines neutron irradiation in presence of 10B at the accelerator based epithermal neutron source at Budker Institute of Nuclear Physics was conducted. Cells were incubated with different concentrations of 10B labeled boronophenylalanine to the growth medium for 24 hours. The samples were placed in polymethyl methacrylate phantom on a depth of 36 mm under the neutron-generating target with following irradiation of the cells by neutron flux. Colony forming assay was performed to evaluate clonogenic capabilities of irradiated glioma and melanoma cells. I t showed that 10B increased by irradiation with neutron beam, leads to decrease in cell survival. It showed that the cells surviving decreased with increasing of 10B after irradiation with neutron beam. Concentrations of 10B in U251 and SK-Mel28 cells were detected with atomic emission spectrometer ICPE-9820 (Shimadzu, Japan) that of confirm the dependence of boron accumulation and cell death. The report presents and discusses the results of this investigation.

UR - https://elibrary.ru/item.asp?id=47962317&pff=1

M3 - Conference contribution

SP - 179

BT - 19th International Congress on Neutron Capture Therapy Granada, Spain, September 27th - October 1st, 2021. Book of Abstacts

PB - International Atomic Energy Agency

CY - Вена

ER -