TY - CHAP

T1 - Impact of PARP1, PARP2 & PARP3 on the Base Excision Repair of Nucleosomal DNA

AU - Kutuzov, M. M.

AU - Belousova, E. A.

AU - Ilina, E. S.

AU - Lavrik, O. I.

N1 - Funding Information: Acknowledgements This work was supported by RSF project № 17-74-20075. Publisher Copyright: © 2020, Springer Nature Switzerland AG. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/5/8

Y1 - 2020/5/8

N2 - DNA is constantly attacked by different damaging agents; therefore, it requires frequent repair. On the one hand, the base excision repair (BER) system is responsible for the repair of the most frequent DNA lesions. On the other hand, the formation of poly(ADP-ribose) is one of the main DNA damage response reactions that is catalysed by members of the PARP family. PARP1, which belongs to the PARP family and performs approximately 90% of PAR synthesis in cells, could be considered a main regulator of the BER process. Most of the experimental data concerning BER investigation have been obtained using naked DNA. However, in the context of the eukaryotic cell, DNA is compacted in the nucleus, and the lowest compaction level is represented by the nucleosome. Thus, the organization of DNA into the nucleosome impacts the DNA-protein interactions that are involved in BER processes. Poly(ADP-ribosyl)ation (PARylation) is thought to regulate the initiation of the BER process at the chromatin level. In this review, we focus on the mechanisms involved in BER in the nucleosomal context and the potential effect of PARylation, which is catalysed by DNA-dependent PARP1, PARP2 and PARP3 proteins, on this process.

AB - DNA is constantly attacked by different damaging agents; therefore, it requires frequent repair. On the one hand, the base excision repair (BER) system is responsible for the repair of the most frequent DNA lesions. On the other hand, the formation of poly(ADP-ribose) is one of the main DNA damage response reactions that is catalysed by members of the PARP family. PARP1, which belongs to the PARP family and performs approximately 90% of PAR synthesis in cells, could be considered a main regulator of the BER process. Most of the experimental data concerning BER investigation have been obtained using naked DNA. However, in the context of the eukaryotic cell, DNA is compacted in the nucleus, and the lowest compaction level is represented by the nucleosome. Thus, the organization of DNA into the nucleosome impacts the DNA-protein interactions that are involved in BER processes. Poly(ADP-ribosyl)ation (PARylation) is thought to regulate the initiation of the BER process at the chromatin level. In this review, we focus on the mechanisms involved in BER in the nucleosomal context and the potential effect of PARylation, which is catalysed by DNA-dependent PARP1, PARP2 and PARP3 proteins, on this process.

KW - Base excision repair

KW - DNA damge response

KW - NCP

KW - Nucleosome core particle

KW - PARP1

KW - PARP2

KW - PARP3

KW - Poly(ADP-ribosyl)ation

KW - Poly(ADP-ribose) Polymerases/metabolism

KW - Humans

KW - DNA/genetics

KW - Animals

KW - DNA Repair

KW - DNA Damage

KW - Nucleosomes/genetics

UR - http://www.scopus.com/inward/record.url?scp=85084394151&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/fc641cfe-4881-3dc6-aee2-64fc7e505292/

U2 - 10.1007/978-3-030-41283-8_4

DO - 10.1007/978-3-030-41283-8_4

M3 - Chapter

C2 - 32383115

AN - SCOPUS:85084394151

SN - 978-3-030-41282-1

SN - 978-3-030-41285-2

VL - 1241

T3 - Advances in experimental medicine and biology

SP - 47

EP - 57

BT - Mechanisms of Genome Protection and Repair

A2 - Zharkov, Dmitry O.

PB - Springer, Cham

ER -