Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Impact of Double-Stranded RNA Internalization on Hematopoietic Progenitors and Krebs-2 Cells and Mechanism. / Ritter, Genrikh S.; Proskurina, Anastasia S.; Meschaninova, Maria I. и др.
в: International Journal of Molecular Sciences, Том 24, № 5, 4858, 02.03.2023.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Impact of Double-Stranded RNA Internalization on Hematopoietic Progenitors and Krebs-2 Cells and Mechanism
AU - Ritter, Genrikh S.
AU - Proskurina, Anastasia S.
AU - Meschaninova, Maria I.
AU - Potter, Ekaterina A.
AU - Petrova, Daria D.
AU - Ruzanova, Vera S.
AU - Dolgova, Evgeniya V.
AU - Kirikovich, Svetlana S.
AU - Levites, Evgeniy V.
AU - Efremov, Yaroslav R.
AU - Nikolin, Valeriy P.
AU - Popova, Nelly A.
AU - Venyaminova, Aliya G.
AU - Taranov, Oleg S.
AU - Ostanin, Alexandr A.
AU - Chernykh, Elena R.
AU - Kolchanov, Nikolay A.
AU - Bogachev, Sergey S.
N1 - This research was funded by the Russian Ministry of Science and High Education via the Institute of Cytology and Genetics [State Budget Project No FWNR-2022-0016]. The authors are grateful for the additional financial help for Inga N. Zaitseva and Aleksey A. Purtov. Публикация для корректировки.
PY - 2023/3/2
Y1 - 2023/3/2
N2 - It is well-established that double-stranded RNA (dsRNA) exhibits noticeable radioprotective and radiotherapeutic effects. The experiments conducted in this study directly demonstrated that dsRNA was delivered into the cell in its native form and that it induced hematopoietic progenitor proliferation. The 68 bp synthetic dsRNA labeled with 6-carboxyfluorescein (FAM) was internalized into mouse hematopoietic progenitors, c-Kit+ (a marker of long-term hematopoietic stem cells) cells and CD34+ (a marker of short-term hematopoietic stem cells and multipotent progenitors) cells. Treating bone marrow cells with dsRNA stimulated the growth of colonies, mainly cells of the granulocyte–macrophage lineage. A total of 0.8% of Krebs-2 cells internalized FAM-dsRNA and were simultaneously CD34+ cells. dsRNA in its native state was delivered into the cell, where it was present without any signs of processing. dsRNA binding to a cell was independent of cell charge. dsRNA internalization was related to the receptor-mediated process that requires energy from ATP. Synthetic dsRNA did not degrade in the bloodstream for at least 2 h. Hematopoietic precursors that had captured dsRNA reinfused into the bloodstream and populated the bone marrow and spleen. This study, for the first time, directly proved that synthetic dsRNA is internalized into a eukaryotic cell via a natural mechanism.
AB - It is well-established that double-stranded RNA (dsRNA) exhibits noticeable radioprotective and radiotherapeutic effects. The experiments conducted in this study directly demonstrated that dsRNA was delivered into the cell in its native form and that it induced hematopoietic progenitor proliferation. The 68 bp synthetic dsRNA labeled with 6-carboxyfluorescein (FAM) was internalized into mouse hematopoietic progenitors, c-Kit+ (a marker of long-term hematopoietic stem cells) cells and CD34+ (a marker of short-term hematopoietic stem cells and multipotent progenitors) cells. Treating bone marrow cells with dsRNA stimulated the growth of colonies, mainly cells of the granulocyte–macrophage lineage. A total of 0.8% of Krebs-2 cells internalized FAM-dsRNA and were simultaneously CD34+ cells. dsRNA in its native state was delivered into the cell, where it was present without any signs of processing. dsRNA binding to a cell was independent of cell charge. dsRNA internalization was related to the receptor-mediated process that requires energy from ATP. Synthetic dsRNA did not degrade in the bloodstream for at least 2 h. Hematopoietic precursors that had captured dsRNA reinfused into the bloodstream and populated the bone marrow and spleen. This study, for the first time, directly proved that synthetic dsRNA is internalized into a eukaryotic cell via a natural mechanism.
KW - Krebs-2 cancer stem cells
KW - dsRNA
KW - hematopoietic progenitors
KW - internalization
KW - stimulation of colony growth
KW - RNA, Double-Stranded/pharmacology
KW - Cells, Cultured
KW - Hematopoietic Stem Cells/metabolism
KW - Animals
KW - Antigens, CD34/metabolism
KW - Bone Marrow/metabolism
KW - Mice
KW - Bone Marrow Cells/metabolism
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85149965037&origin=inward&txGid=647096703d01334b0fd1e82737a62515
UR - https://www.mendeley.com/catalogue/a37f4ce3-ccaf-3df2-86df-0e3723eababe/
U2 - 10.3390/ijms24054858
DO - 10.3390/ijms24054858
M3 - Article
C2 - 36902311
VL - 24
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 5
M1 - 4858
ER -
ID: 59242607