TY - JOUR

T1 - Hydroxyapatite Double Substituted with Zinc and Silicate Ions: Possibility of Mechanochemical Synthesis and In Vitro Properties

AU - Makarova, Svetlana V

AU - Bulina, Natalia V

AU - Golubeva, Yuliya A

AU - Klyushova, Lyubov S

AU - Dumchenko, Natalya B

AU - Shatskaya, Svetlana S

AU - Ishchenko, Arcady V

AU - Khvostov, Mikhail V

AU - Dudina, Dina V

N1 - Funding: This research was carried out within a state assignment to the Institute of Solid State Chemistry and Mechanochemistry SB RAS (project No. 121032500064-8). The study of biocompatibility and cytotoxicity was performed using the equipment of the Center for Collective Use “Proteomic Analysis”, supported by funding from the Ministry of Science and Higher Education of the Russian Federation (agreement No. 075-15-2021-691).

PY - 2023/2/7

Y1 - 2023/2/7

N2 - In this study, the mechanochemical synthesis of substituted hydroxyapatite (HA) containing zinc and silicon ions having a chemical formula of Ca10-xZnx(PO4)6-x(SiO4)x(OH)2-x, where x = 0.2, 0.6, 1.0, 1.5, and 2.0, was carried out. The synthesized materials were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, and inductively coupled plasma spectroscopy. We found that HA co-substituted with zinc and silicate formed up to x = 1.0. At higher concentrations of the substituents, the formation of large amounts of an amorphous phase was observed. The cytotoxicity and biocompatibility of the co-substituted HA was studied in vitro on Hek293 and MG-63 cell lines. The HA co-substituted with zinc and silicate demonstrated high biocompatibility; the lowest cytotoxicity was observed at x = 0.2. For this composition, good proliferation of MG-63 osteoblast-like cells and an increased solubility compared with that of HA were detected. These properties allow us to recommend the synthesized material for medical applications, namely, for the restoration of bone tissue and manufacture of biodegradable implants.

AB - In this study, the mechanochemical synthesis of substituted hydroxyapatite (HA) containing zinc and silicon ions having a chemical formula of Ca10-xZnx(PO4)6-x(SiO4)x(OH)2-x, where x = 0.2, 0.6, 1.0, 1.5, and 2.0, was carried out. The synthesized materials were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, and inductively coupled plasma spectroscopy. We found that HA co-substituted with zinc and silicate formed up to x = 1.0. At higher concentrations of the substituents, the formation of large amounts of an amorphous phase was observed. The cytotoxicity and biocompatibility of the co-substituted HA was studied in vitro on Hek293 and MG-63 cell lines. The HA co-substituted with zinc and silicate demonstrated high biocompatibility; the lowest cytotoxicity was observed at x = 0.2. For this composition, good proliferation of MG-63 osteoblast-like cells and an increased solubility compared with that of HA were detected. These properties allow us to recommend the synthesized material for medical applications, namely, for the restoration of bone tissue and manufacture of biodegradable implants.

KW - biocompatibility

KW - co-substitution

KW - cytotoxicity

KW - doping

KW - hydroxyapatite

KW - silicone

KW - zinc

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85149254132&origin=inward&txGid=3e7b3f556436ac9417f5be4cdf3f7005

UR - https://www.mendeley.com/catalogue/061ab1e3-95c1-3881-adfd-ef60a19ba3e5/

U2 - 10.3390/ma16041385

DO - 10.3390/ma16041385

M3 - Article

C2 - 36837015

VL - 16

JO - Materials

JF - Materials

SN - 1996-1944

IS - 4

M1 - 1385

ER -