TY - JOUR

T1 - Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus

AU - Novikova, Tatiana S

AU - Ermakov, Evgeny A

AU - Kostina, Elena V

AU - Sinyakov, Alexander N

AU - Sizikov, Alexey E

AU - Nevinsky, Georgy A

AU - Buneva, Valentina N

N1 - This work was funded by the Russian Science Foundation [grant number 23-15-00357 (the main part of the work on anti-DNA antibody analysis)] and the Russian state-funded project for ICBFM SB RAS [grant numbers 121031300042-1 (part of the work on ODN synthesis and microarray printing) and 121031300041-4 (part of the research on DNase I)].

PY - 2023/12

Y1 - 2023/12

N2 - Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in solution by catalytic anti-DNA antibodies from SLE patients. It has been shown that IgG antibodies from SLE patients hydrolyze ODNs more effectively both in solution and on the surface, compared to IgG from healthy individuals. The data obtained indicate a more efficient hydrolysis of ODNs in solution than immobilized ODNs on the surface. In addition, differences in the specificity of recognition and hydrolysis of certain ODNs by anti-DNA antibodies were revealed, indicating the formation of autoantibodies to specific DNA motifs in SLE. The data obtained expand our understanding of the role of anti-DNA antibodies in SLE. Differences in the recognition and hydrolysis of surface-tethered and dissolved ODNs need to be considered in DNA microarray applications.

AB - Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in solution by catalytic anti-DNA antibodies from SLE patients. It has been shown that IgG antibodies from SLE patients hydrolyze ODNs more effectively both in solution and on the surface, compared to IgG from healthy individuals. The data obtained indicate a more efficient hydrolysis of ODNs in solution than immobilized ODNs on the surface. In addition, differences in the specificity of recognition and hydrolysis of certain ODNs by anti-DNA antibodies were revealed, indicating the formation of autoantibodies to specific DNA motifs in SLE. The data obtained expand our understanding of the role of anti-DNA antibodies in SLE. Differences in the recognition and hydrolysis of surface-tethered and dissolved ODNs need to be considered in DNA microarray applications.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85180667852&origin=inward&txGid=c3c7f8c19fbf404d604087c8d12250a4

U2 - 10.3390/cimb45120617

DO - 10.3390/cimb45120617

M3 - Article

C2 - 38132463

VL - 45

SP - 9887

EP - 9903

JO - Current issues in molecular biology

JF - Current issues in molecular biology

SN - 1467-3037

IS - 12

ER -