Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Heteromorphism of "homomorphic" sex chromosomes in two anole species (Squamata, Dactyloidae) revealed by synaptonemal complex analysis. / Lisachov, Artem P.; Trifonov, Vladimir A.; Giovannotti, Massimo и др.
в: Cytogenetic and Genome Research, Том 151, № 2, 06.2017, стр. 89-95.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Heteromorphism of "homomorphic" sex chromosomes in two anole species (Squamata, Dactyloidae) revealed by synaptonemal complex analysis
AU - Lisachov, Artem P.
AU - Trifonov, Vladimir A.
AU - Giovannotti, Massimo
AU - Ferguson-Smith, Malcolm A.
AU - Borodin, Pavel M.
N1 - Publisher Copyright: © 2017 S. Karger AG, Basel.
PY - 2017/6
Y1 - 2017/6
N2 - Iguanians (Pleurodonta) are one of the reptile lineages that, like birds and mammals, have sex chromosomes of ancient origin. In most iguanians these are microchromosomes, making a distinction between the X and Y as well as between homeologous sex chromosomes in other species difficult. Meiotic chromosome analysis may be used to elucidate their differentiation, because meiotic prophase chromosomes are longer and less condensed than metaphase chromosomes, and the homologues are paired with each other, revealing minor heteromorphisms. Using electron and fluorescent microscopy of surface spread synaptonemal complexes (SCs) and immunolocalization of the proteins of the SC (SYCP3), the centromere, and recombination nodules (MLH1), we examined sex chromosome synapsis and recombination in 2 species of anoles (Dactyloidae), Anolis carolinensis and Deiroptyx coelestinus, in which the sex chromosomes represent the ancestral condition of iguanians. We detected clear differences in size between the anole X and Y microchromosomes and found an interspecies difference in the localization of the pseudoautosomal region. Our results show that the apparent homomorphy of certain reptile sex chromosome systems can hide a cryptic differentiation, which potentially may influence the evolution of sexual dimorphism and speciation.
AB - Iguanians (Pleurodonta) are one of the reptile lineages that, like birds and mammals, have sex chromosomes of ancient origin. In most iguanians these are microchromosomes, making a distinction between the X and Y as well as between homeologous sex chromosomes in other species difficult. Meiotic chromosome analysis may be used to elucidate their differentiation, because meiotic prophase chromosomes are longer and less condensed than metaphase chromosomes, and the homologues are paired with each other, revealing minor heteromorphisms. Using electron and fluorescent microscopy of surface spread synaptonemal complexes (SCs) and immunolocalization of the proteins of the SC (SYCP3), the centromere, and recombination nodules (MLH1), we examined sex chromosome synapsis and recombination in 2 species of anoles (Dactyloidae), Anolis carolinensis and Deiroptyx coelestinus, in which the sex chromosomes represent the ancestral condition of iguanians. We detected clear differences in size between the anole X and Y microchromosomes and found an interspecies difference in the localization of the pseudoautosomal region. Our results show that the apparent homomorphy of certain reptile sex chromosome systems can hide a cryptic differentiation, which potentially may influence the evolution of sexual dimorphism and speciation.
KW - Anolis
KW - Deiroptyx
KW - Meiosis
KW - Recombination
KW - Reptilia
KW - Synaptonemal complex
KW - Lizards/genetics
KW - Synaptonemal Complex/genetics
KW - In Situ Hybridization, Fluorescence
KW - Male
KW - Chromosomal Proteins, Non-Histone/genetics
KW - Sex Characteristics
KW - Sex Chromosomes
KW - Animals
KW - Recombination, Genetic
KW - Karyotype
KW - Microscopy, Fluorescence
KW - EVOLUTIONARY DYNAMICS
KW - LACERTA-AGILIS
KW - PATTERN
KW - CHICKEN
KW - DIVERGENCE
KW - MAMMALS
KW - RECOMBINATION
KW - SAND LIZARD
KW - GENOME
KW - GREEN ANOLE
UR - http://www.scopus.com/inward/record.url?scp=85015835529&partnerID=8YFLogxK
U2 - 10.1159/000460829
DO - 10.1159/000460829
M3 - Article
C2 - 28315859
AN - SCOPUS:85015835529
VL - 151
SP - 89
EP - 95
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 2
ER -
ID: 8975546