TY - JOUR

T1 - Halamphora kolbei (Aleem) Álvarez-Blanco et S. Blanco 2014, A Rare Diatom from the Black Sea: Morphological Observations and Revised Description with Biochemical Composition

AU - Blaginina, Anastasiia Andreevna

AU - Balycheva, Daria Sergeevna

AU - Kezlya, Elena Mikhailovna

AU - Zheleznova, Svetlana Nikolaevna

AU - Gevorgiz, Ruslan Georgievich

AU - Miroshnichenko, Ekaterina Sergeevna

AU - Kulikovskiy, Maxim Sergeevich

AU - Ryabushko, Larisa Ivanovna

N1 - This research was supported by the Ministry of Science and Higher Education of the Russian Federation within the state assignment 124022400152-1 (“Comprehensive study of the mechanisms of functioning of marine biotechnological complexes for obtaining biologically active substances from aquatic organisms”) and by the Russian Science Foundation (Grant № 25-79-30002). Molecular data were obtained with financial support from the framework of state assignment of the Ministry of Science and Higher Education of the Russian Federation (theme 126012015839-6).

PY - 2026/2/27

Y1 - 2026/2/27

N2 - The species Halamphora kolbei is a rarely reported diatom, and information on its distribution and characteristics is limited. The original taxonomic description of this species is inaccurate and insufficient for identification, as the available literature contains scarce and scattered data. In the present study, we summarised the available data on this species and provided a detailed description of the strain isolated from Sevastopol Bay of the Black Sea. Findings from a phylogenetic analysis of two genes, 18S rRNA and rbcL, are presented. The process of isolation, identification and establishment of a monoculture of the H. kolbei is described in detail. In addition, the productivity and biochemical characteristics were evaluated to assess the biotechnological potential of the strain. The growth rate, biomass productivity and protein, carbohydrate, lipid and fatty acid composition of this species were determined in this study, under controlled conditions. The high content of C14–C16 acids in H. kolbei has been revealed to make it a promising source of eicosapentaenoic acid. The detailed description of the species, its photographs and phylogeny, as well as additional morphological keys presented in the paper, will enable scientists around the world to study this species as a promising biological resource.

AB - The species Halamphora kolbei is a rarely reported diatom, and information on its distribution and characteristics is limited. The original taxonomic description of this species is inaccurate and insufficient for identification, as the available literature contains scarce and scattered data. In the present study, we summarised the available data on this species and provided a detailed description of the strain isolated from Sevastopol Bay of the Black Sea. Findings from a phylogenetic analysis of two genes, 18S rRNA and rbcL, are presented. The process of isolation, identification and establishment of a monoculture of the H. kolbei is described in detail. In addition, the productivity and biochemical characteristics were evaluated to assess the biotechnological potential of the strain. The growth rate, biomass productivity and protein, carbohydrate, lipid and fatty acid composition of this species were determined in this study, under controlled conditions. The high content of C14–C16 acids in H. kolbei has been revealed to make it a promising source of eicosapentaenoic acid. The detailed description of the species, its photographs and phylogeny, as well as additional morphological keys presented in the paper, will enable scientists around the world to study this species as a promising biological resource.

KW - Black Sea

KW - Halamphora kolbei

KW - benthic diatom

KW - biochemical composition

KW - bioprospecting

KW - phylogenetic analysis

UR - https://www.scopus.com/pages/publications/105032782514

UR - https://www.elibrary.ru/item.asp?id=89148246

UR - https://www.mendeley.com/catalogue/6a2ef2ee-5d46-3d1d-b7a1-7b007ec446ac/

U2 - 10.3390/biology15050394

DO - 10.3390/biology15050394

M3 - Article

VL - 15

JO - Biology

JF - Biology

SN - 2079-7737

IS - 5

M1 - 394

ER -