TY - JOUR

T1 - Global LC-MS/MS targeted metabolomics using a combination of HILIC and RP LC separation modes on an organic monolithic column based on 1-vinyl-1,2,4-triazole

AU - Basov, Nikita V

AU - Rogachev, Artem D

AU - Aleshkova, Maria A

AU - Gaisler, Evgeny V

AU - Sotnikova, Yulia S

AU - Patrushev, Yuri V

AU - Tolstikova, Tatiana G

AU - Yarovaya, Olga I

AU - Pokrovsky, Andrey G

AU - Salakhutdinov, Nariman F

N1 - Funding: The study was supported by Russian Science Foundation (Moscow, Russia) grant No. 22-23-01068. Copyright © 2023 Elsevier B.V. All rights reserved.

PY - 2024/1/15

Y1 - 2024/1/15

N2 - The paper presents an LC-MS/MS-based approach to targeted screening of both polar and non-polar metabolites using a synthesized monolithic column which is a copolymer of styrene, divinylbenzene, and 1-vinyl-1,2,4-triazole. It was shown that this column in combination with eluents 20 mM (NH4)2CO3 + NH3 (pH = 9.8, eluent A) and ACN (eluent B) allows for separation of metabolites of different nature in two modes, HILIC and RP LC, and these methods are mutually complementary. A combination of analyses based on these two modes was proposed, allowing detection of about 400 metabolites in a total time of less than 30 min. Comparison of the developed method with those utilizing commercially available columns with sorbents of various types showed that it could provide a broader metabolite coverage. Using the developed approach, metabolomic screening of dried blood spots samples of mice exposed with X-ray was performed, and metabolites that could be considered as possible markers of irradiation exposure and organ tissue damage were detected. Analysis of marker metabolites revealed metabolic pathways that were altered by radiation exposure. Comparison of the results with literature data showed the effectiveness of the developed metabolomic screening approach.

AB - The paper presents an LC-MS/MS-based approach to targeted screening of both polar and non-polar metabolites using a synthesized monolithic column which is a copolymer of styrene, divinylbenzene, and 1-vinyl-1,2,4-triazole. It was shown that this column in combination with eluents 20 mM (NH4)2CO3 + NH3 (pH = 9.8, eluent A) and ACN (eluent B) allows for separation of metabolites of different nature in two modes, HILIC and RP LC, and these methods are mutually complementary. A combination of analyses based on these two modes was proposed, allowing detection of about 400 metabolites in a total time of less than 30 min. Comparison of the developed method with those utilizing commercially available columns with sorbents of various types showed that it could provide a broader metabolite coverage. Using the developed approach, metabolomic screening of dried blood spots samples of mice exposed with X-ray was performed, and metabolites that could be considered as possible markers of irradiation exposure and organ tissue damage were detected. Analysis of marker metabolites revealed metabolic pathways that were altered by radiation exposure. Comparison of the results with literature data showed the effectiveness of the developed metabolomic screening approach.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85171749087&origin=inward&txGid=db8d95ef1f7c69df1aff915a404fd7db

UR - https://www.mendeley.com/catalogue/9822040c-864e-370c-b0eb-863537b13027/

U2 - 10.1016/j.talanta.2023.125168

DO - 10.1016/j.talanta.2023.125168

M3 - Article

C2 - 37708770

VL - 267

JO - Talanta

JF - Talanta

SN - 0039-9140

M1 - 125168

ER -