Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Genetic diversity of brucella melitensis in kazakhstan in relation to world-wide diversity. / Shevtsova, Elena; Vergnaud, Gilles; Shevtsov, Alexandr и др.
в: Frontiers in Microbiology, Том 10, № AUG, 1897, 13.08.2019, стр. 1897.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Genetic diversity of brucella melitensis in kazakhstan in relation to world-wide diversity
AU - Shevtsova, Elena
AU - Vergnaud, Gilles
AU - Shevtsov, Alexandr
AU - Shustov, Alexandr
AU - Berdimuratova, Kalysh
AU - Mukanov, Kasim
AU - Syzdykov, Marat
AU - Kuznetsov, Andrey
AU - Lukhnova, Larissa
AU - Izbanova, Uinkul
AU - Filipenko, Maxim
AU - Ramankulov, Yerlan
PY - 2019/8/13
Y1 - 2019/8/13
N2 - We describe the genetic diversity of 1327 Brucella strains from human patients in Kazakhstan using multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA). All strains were assigned to the Brucella melitensis East Mediterranean group and clustered into 16 MLVA11 genotypes, nine of which are reported for the first time. MLVA11 genotype 116 predominates (86.8%) and is present all over Kazakhstan indicating existence and temporary preservation of a “founder effect” among B. melitensis strains circulating in Central Eurasia. The diversity pattern observed in humans is highly similar to the pattern previously reported in animals. The diversity observed by MLVA suggested that the epidemiological status of brucellosis in Kazakhstan is the result of the introduction of a few lineages, which have subsequently diversified at the most unstable tandem repeat loci. This investigation will allow to select the most relevant strains for testing these hypotheses via whole genome sequencing and to subsequently adjust the genotyping scheme to the Kazakhstan epidemiological situation.
AB - We describe the genetic diversity of 1327 Brucella strains from human patients in Kazakhstan using multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA). All strains were assigned to the Brucella melitensis East Mediterranean group and clustered into 16 MLVA11 genotypes, nine of which are reported for the first time. MLVA11 genotype 116 predominates (86.8%) and is present all over Kazakhstan indicating existence and temporary preservation of a “founder effect” among B. melitensis strains circulating in Central Eurasia. The diversity pattern observed in humans is highly similar to the pattern previously reported in animals. The diversity observed by MLVA suggested that the epidemiological status of brucellosis in Kazakhstan is the result of the introduction of a few lineages, which have subsequently diversified at the most unstable tandem repeat loci. This investigation will allow to select the most relevant strains for testing these hypotheses via whole genome sequencing and to subsequently adjust the genotyping scheme to the Kazakhstan epidemiological situation.
KW - Brucella melitensis
KW - Genetic diversity
KW - Genotyping
KW - Kazakhstan
KW - Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA)
KW - genetic diversity
KW - MULTIPLEX PCR
KW - ASSAY
KW - ASIA
KW - genotyping
KW - multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA)
UR - http://www.scopus.com/inward/record.url?scp=85071928031&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2019.01897
DO - 10.3389/fmicb.2019.01897
M3 - Article
C2 - 31456793
AN - SCOPUS:85071928031
VL - 10
SP - 1897
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - AUG
M1 - 1897
ER -
ID: 21469290