Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Generation of Induced Pluripotent Stem Cell Line iTAF15Xsk4 from Fibroblasts of a Patient with Microdeletion at Xq24. / Pristyazhnyuk, I. E.; Meshcheryakov, N. I.; Nikitina, T. V. и др.
в: Russian Journal of Developmental Biology, Том 54, № 6, 07.03.2024, стр. 358-364.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Generation of Induced Pluripotent Stem Cell Line iTAF15Xsk4 from Fibroblasts of a Patient with Microdeletion at Xq24
AU - Pristyazhnyuk, I. E.
AU - Meshcheryakov, N. I.
AU - Nikitina, T. V.
AU - Kashevarova, A. A.
AU - Fedotov, D. A.
AU - Tolmacheva, E. N.
AU - Minaycheva, L. I.
AU - Nazarenko, L. P.
AU - Lebedev, I. N.
AU - Menzorov, A. G.
N1 - Generation of Induced Pluripotent Stem Cell Line iTAF15Xsk4 from Fibroblasts of a Patient with Microdeletion at Xq24 / I. E. Pristyazhnyuk, N. I. Meshcheryakov, T. V. Nikitina [et al.] // Russian Journal of Developmental Biology. – 2023. – Vol. 54, No. 6. – P. 358-364. – DOI 10.1134/s1062360423060073.
PY - 2024/3/7
Y1 - 2024/3/7
N2 - Differentiation of induced pluripotent stem cells (iPSCs) from patients and healthy donors allows in vitro study of genetic disorders. The authors have previously reported a clinical case of recurrent pregnancy loss in a patient with skewed X-chromosome inactivation in peripheral blood lymphocytes, endometrium, and buccal epithelium. A 239 kb microdeletion at Xq24 that affected eight genes, including UBE2A, has been found. In this work, an iPSC line iTAF15Xsk4 was produced from the patient’s skin fibroblasts using nonintegrating episomal vectors. The iPSC line had a normal karyotype, expressed pluripotency markers, and expressed markers of all three germ layers upon differentiation in embryoid bodies. This cell line could be used for the UBE2A deficiency syndrome study.
AB - Differentiation of induced pluripotent stem cells (iPSCs) from patients and healthy donors allows in vitro study of genetic disorders. The authors have previously reported a clinical case of recurrent pregnancy loss in a patient with skewed X-chromosome inactivation in peripheral blood lymphocytes, endometrium, and buccal epithelium. A 239 kb microdeletion at Xq24 that affected eight genes, including UBE2A, has been found. In this work, an iPSC line iTAF15Xsk4 was produced from the patient’s skin fibroblasts using nonintegrating episomal vectors. The iPSC line had a normal karyotype, expressed pluripotency markers, and expressed markers of all three germ layers upon differentiation in embryoid bodies. This cell line could be used for the UBE2A deficiency syndrome study.
UR - https://www.mendeley.com/catalogue/5a43dc68-ed1a-39aa-883b-990bee26d731/
UR - https://www.elibrary.ru/item.asp?id=65177199
U2 - 10.1134/s1062360423060073
DO - 10.1134/s1062360423060073
M3 - Article
VL - 54
SP - 358
EP - 364
JO - Russian Journal of Developmental Biology
JF - Russian Journal of Developmental Biology
SN - 1062-3604
IS - 6
ER -
ID: 68319573