Standard

Fluorescence-free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets. / Litvinenko, A. L.; Moskalensky, A. E.; Karmadonova, N. A. и др.

в: Cytometry Part A, Том 89, № 11, 01.11.2016, стр. 1010-1016.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Litvinenko, AL, Moskalensky, AE, Karmadonova, NA, Nekrasov, VM, Strokotov, DI, Konokhova, AI, Yurkin, MA, Pokushalov, EA, Chernyshev, AV & Maltsev, VP 2016, 'Fluorescence-free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets', Cytometry Part A, Том. 89, № 11, стр. 1010-1016. https://doi.org/10.1002/cyto.a.23003

APA

Litvinenko, A. L., Moskalensky, A. E., Karmadonova, N. A., Nekrasov, V. M., Strokotov, D. I., Konokhova, A. I., Yurkin, M. A., Pokushalov, E. A., Chernyshev, A. V., & Maltsev, V. P. (2016). Fluorescence-free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets. Cytometry Part A, 89(11), 1010-1016. https://doi.org/10.1002/cyto.a.23003

Vancouver

Litvinenko AL, Moskalensky AE, Karmadonova NA, Nekrasov VM, Strokotov DI, Konokhova AI и др. Fluorescence-free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets. Cytometry Part A. 2016 нояб. 1;89(11):1010-1016. doi: 10.1002/cyto.a.23003

Author

Litvinenko, A. L. ; Moskalensky, A. E. ; Karmadonova, N. A. и др. / Fluorescence-free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets. в: Cytometry Part A. 2016 ; Том 89, № 11. стр. 1010-1016.

BibTeX

@article{2fb42c8689b34a809f482f65b42035f8,
title = "Fluorescence-free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets",
abstract = "Whereas commercially available hematological analyzers measure volume of individual platelets, angle-resolved light-scattering provides unique ability to additionally measure their shape index. We utilized the scanning flow cytometer to measure light-scattering profiles (LSPs) of individual platelets taken from 16 healthy donors and the solution of the inverse light-scattering problem to retrieve the volume and shape index of each platelet. In normal conditions, the platelet shape index distribution (PSID) demonstrates three peaks, which relate to resting, partially activated, and fully activated platelets. We developed an algorithm, based on fitting PSID by a sum of three peak functions, to determine the percentage, mean platelet shape index, and distribution width of each platelet fraction. In total, this method gives eight additional parameters of platelet morphology and function to be used in clinical hematological analysis. We also stimulated the platelets with adenosine diphosphate (ADP) and measured the dependence of equilibrium PSID, including the total percentage of activated platelets, on ADP concentration.",
keywords = "flow cytometry, light scattering, platelet activation, platelet shape",
author = "Litvinenko, {A. L.} and Moskalensky, {A. E.} and Karmadonova, {N. A.} and Nekrasov, {V. M.} and Strokotov, {D. I.} and Konokhova, {A. I.} and Yurkin, {M. A.} and Pokushalov, {E. A.} and Chernyshev, {A. V.} and Maltsev, {V. P.}",
year = "2016",
month = nov,
day = "1",
doi = "10.1002/cyto.a.23003",
language = "English",
volume = "89",
pages = "1010--1016",
journal = "Cytometry. Part A : the journal of the International Society for Analytical Cytology",
issn = "1552-4922",
publisher = "Wiley-Liss Inc.",
number = "11",

}

RIS

TY - JOUR

T1 - Fluorescence-free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets

AU - Litvinenko, A. L.

AU - Moskalensky, A. E.

AU - Karmadonova, N. A.

AU - Nekrasov, V. M.

AU - Strokotov, D. I.

AU - Konokhova, A. I.

AU - Yurkin, M. A.

AU - Pokushalov, E. A.

AU - Chernyshev, A. V.

AU - Maltsev, V. P.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Whereas commercially available hematological analyzers measure volume of individual platelets, angle-resolved light-scattering provides unique ability to additionally measure their shape index. We utilized the scanning flow cytometer to measure light-scattering profiles (LSPs) of individual platelets taken from 16 healthy donors and the solution of the inverse light-scattering problem to retrieve the volume and shape index of each platelet. In normal conditions, the platelet shape index distribution (PSID) demonstrates three peaks, which relate to resting, partially activated, and fully activated platelets. We developed an algorithm, based on fitting PSID by a sum of three peak functions, to determine the percentage, mean platelet shape index, and distribution width of each platelet fraction. In total, this method gives eight additional parameters of platelet morphology and function to be used in clinical hematological analysis. We also stimulated the platelets with adenosine diphosphate (ADP) and measured the dependence of equilibrium PSID, including the total percentage of activated platelets, on ADP concentration.

AB - Whereas commercially available hematological analyzers measure volume of individual platelets, angle-resolved light-scattering provides unique ability to additionally measure their shape index. We utilized the scanning flow cytometer to measure light-scattering profiles (LSPs) of individual platelets taken from 16 healthy donors and the solution of the inverse light-scattering problem to retrieve the volume and shape index of each platelet. In normal conditions, the platelet shape index distribution (PSID) demonstrates three peaks, which relate to resting, partially activated, and fully activated platelets. We developed an algorithm, based on fitting PSID by a sum of three peak functions, to determine the percentage, mean platelet shape index, and distribution width of each platelet fraction. In total, this method gives eight additional parameters of platelet morphology and function to be used in clinical hematological analysis. We also stimulated the platelets with adenosine diphosphate (ADP) and measured the dependence of equilibrium PSID, including the total percentage of activated platelets, on ADP concentration.

KW - flow cytometry

KW - light scattering

KW - platelet activation

KW - platelet shape

UR - http://www.scopus.com/inward/record.url?scp=84992374660&partnerID=8YFLogxK

U2 - 10.1002/cyto.a.23003

DO - 10.1002/cyto.a.23003

M3 - Article

C2 - 27768824

AN - SCOPUS:84992374660

VL - 89

SP - 1010

EP - 1016

JO - Cytometry. Part A : the journal of the International Society for Analytical Cytology

JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology

SN - 1552-4922

IS - 11

ER -

ID: 23447643