TY - JOUR

T1 - Fitness analysis and transcriptome profiling following repeated mild heat stress of varying frequency in drosophila melanogaster females

AU - Gruntenko, Nataly E.

AU - Karpova, Evgenia K.

AU - Babenko, Vladimir N.

AU - Vasiliev, Gennady V.

AU - Andreenkova, Olga V.

AU - Bobrovskikh, Margarita A.

AU - Menshanov, Petr N.

AU - Babenko, Roman O.

AU - Rauschenbach, Inga Yu

N1 - Funding Information: Funding: This research was funded by the RUSSIAN FOUNDATION FOR BASIC RESEARCH, #19-04-00458. The maintenance of experimental D. melanogaster strains was carried out in the Drosophila collection fund of the Institute of Cytology and Genetics SB RAS and was supported by BP #0259-2021-0016 of the MINISTRY OF SCIENCE AND HIGHER EDUCATION OF THE RUSSIAN FEDERATION. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/12

Y1 - 2021/12

N2 - Understanding how repeated stress affects metabolic and physiological functions in the long run is of crucial importance for evaluating anthropogenic pressure on the environment. We investigated fertility, longevity and metabolism in D. melanogaster females exposed to short-term heat stress (38◦ C, 1 h) repeated daily or weekly. Daily stress was shown to cause a significant decrease in both fertility and longevity, as well as in body mass and triglyceride (fat) content, but a significant increase in trehalose and glucose content. Weekly stress did not affect longevity and carbohydrate metabolism but resulted in a significant decrease in body mass and fat content. Weekly stress did not affect the total level of fertility, despite sharp fertility drops on the exact days of stressing. However, stressing insects weekly, only in the first two weeks after eclosion, caused a significant increase in the total level of fertility. The analysis of differentially expressed genes in the fat bodies and adjacent tissues of researched groups with the use of RNA-Seq profiling revealed changes in signal pathways related to proteolysis/digestion, heat shock protein 23, and in the tightly linked stress-inducible humoral factor Turandot gene network.

AB - Understanding how repeated stress affects metabolic and physiological functions in the long run is of crucial importance for evaluating anthropogenic pressure on the environment. We investigated fertility, longevity and metabolism in D. melanogaster females exposed to short-term heat stress (38◦ C, 1 h) repeated daily or weekly. Daily stress was shown to cause a significant decrease in both fertility and longevity, as well as in body mass and triglyceride (fat) content, but a significant increase in trehalose and glucose content. Weekly stress did not affect longevity and carbohydrate metabolism but resulted in a significant decrease in body mass and fat content. Weekly stress did not affect the total level of fertility, despite sharp fertility drops on the exact days of stressing. However, stressing insects weekly, only in the first two weeks after eclosion, caused a significant increase in the total level of fertility. The analysis of differentially expressed genes in the fat bodies and adjacent tissues of researched groups with the use of RNA-Seq profiling revealed changes in signal pathways related to proteolysis/digestion, heat shock protein 23, and in the tightly linked stress-inducible humoral factor Turandot gene network.

KW - Fertility

KW - Heat stress

KW - Hsp23

KW - Longevity

KW - Metabolism

KW - Transcriptome

KW - Turandot

UR - http://www.scopus.com/inward/record.url?scp=85121583624&partnerID=8YFLogxK

U2 - 10.3390/biology10121323

DO - 10.3390/biology10121323

M3 - Article

C2 - 34943239

AN - SCOPUS:85121583624

VL - 10

JO - Biology

JF - Biology

SN - 2079-7737

IS - 12

M1 - 1323

ER -