Features of Determining Thermodynamic Parameters of Formation of Nucleic Acid Complexes Using Thermal Denaturation with Fluorimetric Signal Detection

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Features of Determining Thermodynamic Parameters of Formation of Nucleic Acid Complexes Using Thermal Denaturation with Fluorimetric Signal Detection. / Shevelev, G. Y.; Kabilov, M. R.; Lomzov, A. A. и др.

в: Russian Journal of Bioorganic Chemistry, Том 45, № 6, 01.11.2019, стр. 684-698.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{0b0ed5369a624ca7bcf869ce275640f9,

title = "Features of Determining Thermodynamic Parameters of Formation of Nucleic Acid Complexes Using Thermal Denaturation with Fluorimetric Signal Detection",

abstract = "In this work, the possibility of precise physicochemical analysis of formation of nucleic acid complexes has been demonstrated using thermal cyclers with fluorescence signal detection. Individual features of heating blocks of various thermal cyclers have been identified. A nonlinear correlation between fluorescence signal intensity and concentration of fluorescent dyes (5,6)-carboxyfluorescein and Rhodamine B has been identified. Using UV and fluorescence melting, the comparison of melting curves at various concentration of full-complementary and mismatched (A/A and C/A) complexes has been conducted. The experimental values of thermodynamic parameters of complex formation for full-complementary and mismatched complexes have been obtained.",

keywords = "fluorimetry, melting temperature of DNA complexes, nucleic acid complexes, oligonucleotides, thermodynamic analysis",

author = "Shevelev, {G. Y.} and Kabilov, {M. R.} and Lomzov, {A. A.} and Dovydenko, {I. S.} and Pyshnyi, {D. V.}",

note = "Publisher Copyright: {\textcopyright} 2019, Pleiades Publishing, Ltd.",

year = "2019",

month = nov,

day = "1",

doi = "10.1134/S1068162019060359",

language = "English",

volume = "45",

pages = "684--698",

journal = "Russian Journal of Bioorganic Chemistry",

issn = "1068-1620",

publisher = "MAIK NAUKA/INTERPERIODICA/SPRINGER",

number = "6",

}

RIS

TY - JOUR

T1 - Features of Determining Thermodynamic Parameters of Formation of Nucleic Acid Complexes Using Thermal Denaturation with Fluorimetric Signal Detection

AU - Shevelev, G. Y.

AU - Kabilov, M. R.

AU - Lomzov, A. A.

AU - Dovydenko, I. S.

AU - Pyshnyi, D. V.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - In this work, the possibility of precise physicochemical analysis of formation of nucleic acid complexes has been demonstrated using thermal cyclers with fluorescence signal detection. Individual features of heating blocks of various thermal cyclers have been identified. A nonlinear correlation between fluorescence signal intensity and concentration of fluorescent dyes (5,6)-carboxyfluorescein and Rhodamine B has been identified. Using UV and fluorescence melting, the comparison of melting curves at various concentration of full-complementary and mismatched (A/A and C/A) complexes has been conducted. The experimental values of thermodynamic parameters of complex formation for full-complementary and mismatched complexes have been obtained.

AB - In this work, the possibility of precise physicochemical analysis of formation of nucleic acid complexes has been demonstrated using thermal cyclers with fluorescence signal detection. Individual features of heating blocks of various thermal cyclers have been identified. A nonlinear correlation between fluorescence signal intensity and concentration of fluorescent dyes (5,6)-carboxyfluorescein and Rhodamine B has been identified. Using UV and fluorescence melting, the comparison of melting curves at various concentration of full-complementary and mismatched (A/A and C/A) complexes has been conducted. The experimental values of thermodynamic parameters of complex formation for full-complementary and mismatched complexes have been obtained.

KW - fluorimetry

KW - melting temperature of DNA complexes

KW - nucleic acid complexes

KW - oligonucleotides

KW - thermodynamic analysis

UR - http://www.scopus.com/inward/record.url?scp=85078603188&partnerID=8YFLogxK

U2 - 10.1134/S1068162019060359

DO - 10.1134/S1068162019060359

M3 - Article

AN - SCOPUS:85078603188

VL - 45

SP - 684

EP - 698

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 6

ER -

ID: 23256507