TY - JOUR

T1 - Facile isolation of lipoprotein-bound myxoxanthophyll and oscillaxanthin from Limnospira (Spirulina) platensis

AU - Gevorgiz, R. G.

AU - Gontcharov, A. A.

AU - Abdullin, S. R.

AU - Nikulin, A. Y.

AU - Kapranova, L. L.

AU - Zheleznova, S. N.

AU - Blaginina, A. A.

AU - Telegina, T. A.

AU - Vechtomova, Y. L.

AU - Maoka, T.

AU - Nekhoroshev, M. V.

N1 - Gevorgiz, R.G., Gontcharov, A.A., Abdullin, S.R. et al. Facile isolation of lipoprotein-bound myxoxanthophyll and oscillaxanthin from Limnospira (Spirulina) platensis. J Appl Phycol (2026). https://doi.org/10.1007/s10811-026-03976-9 The research was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (themes No 124022400152–1; No 124012400285–7; No 126031818944–7). Experiments on L. platensis cultivation in the industrial vortex-type photobioreactor were carried out in the frame of research project supported by the Russian Science Foundation, RSF No 25–79-30002.

PY - 2026/8/3

Y1 - 2026/8/3

N2 - A novel approach to the isolation of commercially valuable carotenoids from cyanobacteria is suggested. The protocol avoids the application of costly column chromatography. As an example, the isolation of myxoxanthophyll and oscillaxanthin from Limnospira (Spirulina) platensis (Gomont) K.R.S.Santos & Hentschke is described. The method is based on the surface-active properties of a unique carotenoid-lipoprotein complex, which accumulates at the chloroform-water interface and can be easily extracted. The complex is composed of myxol 2'-α-L-quinovoside, oscillol 2,2'-di-α-L-quinovoside, and lipoproteins. The mass fraction of carotenoids in the complex was 23 ± 7%. The ratio of the carotenoids in the complex was 63 ± 9% myxoxanthophyll to 27 ± 7% oscillaxanthin. The carotenoids were identified using ultraviolet and visible (UV–Vis) absorption spectroscopy, HPLC analysis, fast atom bombardment mass spectrometry (FAB MS), and proton magnetic resonance spectrometry (1H NMR). The complex was characterized using vibrational infrared spectroscopy (FTIR). Myxoxanthophyll and oscillaxanthin yield from a dry sample of L. platensis was 0.11 ± 0.03% and 0.05 ± 0.02%, respectively, with 40 mg of myxoxanthophyll and 17 mg of oscillaxanthin being isolated from 36.84 g of L. platensis (dry weight). After the carotenoid isolation, further extraction of water-soluble phycobilins from the residual biomass is possible.

AB - A novel approach to the isolation of commercially valuable carotenoids from cyanobacteria is suggested. The protocol avoids the application of costly column chromatography. As an example, the isolation of myxoxanthophyll and oscillaxanthin from Limnospira (Spirulina) platensis (Gomont) K.R.S.Santos & Hentschke is described. The method is based on the surface-active properties of a unique carotenoid-lipoprotein complex, which accumulates at the chloroform-water interface and can be easily extracted. The complex is composed of myxol 2'-α-L-quinovoside, oscillol 2,2'-di-α-L-quinovoside, and lipoproteins. The mass fraction of carotenoids in the complex was 23 ± 7%. The ratio of the carotenoids in the complex was 63 ± 9% myxoxanthophyll to 27 ± 7% oscillaxanthin. The carotenoids were identified using ultraviolet and visible (UV–Vis) absorption spectroscopy, HPLC analysis, fast atom bombardment mass spectrometry (FAB MS), and proton magnetic resonance spectrometry (1H NMR). The complex was characterized using vibrational infrared spectroscopy (FTIR). Myxoxanthophyll and oscillaxanthin yield from a dry sample of L. platensis was 0.11 ± 0.03% and 0.05 ± 0.02%, respectively, with 40 mg of myxoxanthophyll and 17 mg of oscillaxanthin being isolated from 36.84 g of L. platensis (dry weight). After the carotenoid isolation, further extraction of water-soluble phycobilins from the residual biomass is possible.

KW - Limnospira platensis

KW - Цианобактерия

KW - Разделение

KW - Экстракция

KW - Миксоксантофилл

KW - Осциллаксантин

KW - Каротиноиды

KW - Carotenoids

KW - Cyanobacterium

KW - Extraction

KW - Limnospira platensis

KW - Myxoxanthophyll

KW - Oscillaxanthin

KW - Separation

UR - https://www.mendeley.com/catalogue/0e44af7b-658b-3120-b77e-e6efe67b509e/

UR - https://www.scopus.com/pages/publications/105046395800

U2 - 10.1007/s10811-026-03976-9

DO - 10.1007/s10811-026-03976-9

M3 - Article

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

ER -