TY - JOUR

T1 - Expression of palmitoyl transferases in brain structures of mice genetically predisposed to depressive-like behavior

AU - Kondaurova, E. M.

AU - Ilchibaeva, T. V.

AU - Tsybko, A. S.

AU - Ponimaskin, E. G.

AU - Naumenko, V. S.

N1 - Publisher Copyright: © AUTHORS, 2018.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Most G-coupled receptors undergo posttranslational modifications. Among these modifications is S-palmitoylation, carried out by specialized enzymes palmitoyl transferases. Palmitoylation is the covalent attachment of a long-chain fatty acid, palmitate, to cysteine residues. It can influence receptor stability, transportation, and function. Obviously, malfunction of G-protein coupled receptors can cause various psychic disorders, including depression. However, no association between palmitoyl transferases and depressive-like behavior has been found hitherto. There is no information on brain structure specific features of palmitoyl transferase expression either. Here we investigate the expression of ZDHHC5, ZDHHC9, and ZDHHC21 palmitoyl transferases in brain structures of ASC mice with genetic predisposition to depressive-like behavior in comparison with “nondepressive” CBA mice. Several brain region-specific features were detected in the immunodetection of palmitoyl transferase proteins. Western blot of the ZDHHC5 protein in the midbrain revealed two bands at 75 kDa and 55 kDa. Immuno-detection of ZDHHC21 palmitoyl transferase revealed two bands. One of them was visualized at 27 kDa in the frontal cortex and midbrain. The other, at 32 kDa in the hippocampus. Probing for ZDHHC9 also showed two bands in each of the midbrain and hippocampus, at 46 and 41 kDa. However, the expression of all investigated palmitoyl transferases in ASC mice with depressive-like behavior was almost identical to those in CBA mice. Thus, it was the first detection of brain region-specific features of the expression of investigated palmitoyl transferases. However, the study demonstrates that the genetic predisposition to depression-like behavior in ASC mice is not associated with changes in ZDHHC5, ZDHHC9, or ZDHHC21 palmitoyl transferase expression.

AB - Most G-coupled receptors undergo posttranslational modifications. Among these modifications is S-palmitoylation, carried out by specialized enzymes palmitoyl transferases. Palmitoylation is the covalent attachment of a long-chain fatty acid, palmitate, to cysteine residues. It can influence receptor stability, transportation, and function. Obviously, malfunction of G-protein coupled receptors can cause various psychic disorders, including depression. However, no association between palmitoyl transferases and depressive-like behavior has been found hitherto. There is no information on brain structure specific features of palmitoyl transferase expression either. Here we investigate the expression of ZDHHC5, ZDHHC9, and ZDHHC21 palmitoyl transferases in brain structures of ASC mice with genetic predisposition to depressive-like behavior in comparison with “nondepressive” CBA mice. Several brain region-specific features were detected in the immunodetection of palmitoyl transferase proteins. Western blot of the ZDHHC5 protein in the midbrain revealed two bands at 75 kDa and 55 kDa. Immuno-detection of ZDHHC21 palmitoyl transferase revealed two bands. One of them was visualized at 27 kDa in the frontal cortex and midbrain. The other, at 32 kDa in the hippocampus. Probing for ZDHHC9 also showed two bands in each of the midbrain and hippocampus, at 46 and 41 kDa. However, the expression of all investigated palmitoyl transferases in ASC mice with depressive-like behavior was almost identical to those in CBA mice. Thus, it was the first detection of brain region-specific features of the expression of investigated palmitoyl transferases. However, the study demonstrates that the genetic predisposition to depression-like behavior in ASC mice is not associated with changes in ZDHHC5, ZDHHC9, or ZDHHC21 palmitoyl transferase expression.

KW - ASC mice

KW - Depressive-like behavior

KW - Gene expression

KW - Protein level

KW - ZDHHC5

KW - Zdhhc5

KW - ZDHHC9 and ZDHHC21 palmitoyl transferases

KW - Zdhhc9, Zdhhc21 genes

KW - ZDHHC5

KW - ZDHHC9 and ZDHHC21 palmitoyl transferases

KW - Zdhhc5, Zdhhc9, Zdhhc21 genes

KW - gene expression

KW - protein level

KW - ASC mice

KW - depressive-like behavior

KW - 5-HYDROXYTRYPTAMINE(1A) RECEPTOR

KW - SEROTONIN SYSTEM

KW - GENE-EXPRESSION

KW - PROTEIN

KW - 5-HT1A

KW - DISORDERS

KW - ASSAY

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=85052080928&partnerID=8YFLogxK

UR - https://www.elibrary.ru/item.asp?id=35350163

U2 - 10.18699/VJ18.399

DO - 10.18699/VJ18.399

M3 - Article

AN - SCOPUS:85052080928

VL - 22

SP - 593

EP - 599

JO - Вавиловский журнал генетики и селекции

JF - Вавиловский журнал генетики и селекции

SN - 2500-0462

IS - 5

ER -