Standard

Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma. / Boeva, Olga S.; Borisevich, V. I.; Kozlov, V. A. и др.

в: Russian Journal of Immunology, Том 26, № 4, 2023, стр. 527-532.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Boeva, OS, Borisevich, VI, Kozlov, VA, Demina, DV, Sizikov, AE & Pashkina, EA 2023, 'Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma', Russian Journal of Immunology, Том. 26, № 4, стр. 527-532. https://doi.org/10.46235/1028-7221-13919-EON

APA

Boeva, O. S., Borisevich, V. I., Kozlov, V. A., Demina, D. V., Sizikov, A. E., & Pashkina, E. A. (2023). Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma. Russian Journal of Immunology, 26(4), 527-532. https://doi.org/10.46235/1028-7221-13919-EON

Vancouver

Boeva OS, Borisevich VI, Kozlov VA, Demina DV, Sizikov AE, Pashkina EA. Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma. Russian Journal of Immunology. 2023;26(4):527-532. doi: 10.46235/1028-7221-13919-EON

Author

Boeva, Olga S. ; Borisevich, V. I. ; Kozlov, V. A. и др. / Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma. в: Russian Journal of Immunology. 2023 ; Том 26, № 4. стр. 527-532.

BibTeX

@article{13e29237af0b41d2a304697ddd86c13f,
title = "Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma",
abstract = "HLA-E is a minor understudied non-classical HLA genes. HLA-E transcription is revealed in many cell types, especially, in immune cells, e.g., T and B cells monocytes, macrophages. In this work, we evaluated expression of HLA-E on CD8+, CD4+ and CD14+ cells in conditionallу healthy donors and in the patients with bronchial asthma (BA) and rheumatoid arthritis (RA). Peripheral blood mononuclears (PBMNC) were used as initial biomaterial. PBMNC from RA patients (n = 15), BA (n = 11) and healthy donors were separated from peripheral blood in Ficoll-Urographin density gradient (1.077 g/mL). The cells were then stained with fluorochrome-conjugated monoclonal antibodies: anti-CD3-APC, anti-CD4-APC-Cy7, anti-CD-14-FITC, and anti-HLA-E-PerCP/Cy5. The cell phenotype was analyzed by flow cytometry with FACS Canto II (BD Biosciences, USA). We have found an increased expression of HLA-E on CD8+, CD4+Т cells, like as on CD14+ cells (monocytes) in the RA patients, when compared with BA patients. We have also shown significant differences of HLA-E expression on CD8+Т cells between the conditionally healthy donors and RA patients.",
keywords = "allergic diseases, autoimmune diseases, bronchial asthma, major histocompatibility complex molecules, non-classical, rheumatoid arthritis",
author = "Boeva, {Olga S.} and Borisevich, {V. I.} and Kozlov, {V. A.} and Demina, {D. V.} and Sizikov, {A. E.} and Pashkina, {E. A.}",
note = "Боева О.С., Борисевич В.И., Козлов В.А., Демина Д.В., Сизиков А.Э., Пашкина Е.А. Экспрессия «неклассических» молекул главного комплекса гистосовместимости при ревматоидном артрите и бронхиальной астме // Российский иммунологический журнал. - 2023. - Т. 26. - №4. - C. 527-532. Публикация для корректировки.",
year = "2023",
doi = "10.46235/1028-7221-13919-EON",
language = "English",
volume = "26",
pages = "527--532",
journal = "Russian Journal of Immunology",
issn = "2782-7291",
publisher = "Russian Society of Immunology",
number = "4",

}

RIS

TY - JOUR

T1 - Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma

AU - Boeva, Olga S.

AU - Borisevich, V. I.

AU - Kozlov, V. A.

AU - Demina, D. V.

AU - Sizikov, A. E.

AU - Pashkina, E. A.

N1 - Боева О.С., Борисевич В.И., Козлов В.А., Демина Д.В., Сизиков А.Э., Пашкина Е.А. Экспрессия «неклассических» молекул главного комплекса гистосовместимости при ревматоидном артрите и бронхиальной астме // Российский иммунологический журнал. - 2023. - Т. 26. - №4. - C. 527-532. Публикация для корректировки.

PY - 2023

Y1 - 2023

N2 - HLA-E is a minor understudied non-classical HLA genes. HLA-E transcription is revealed in many cell types, especially, in immune cells, e.g., T and B cells monocytes, macrophages. In this work, we evaluated expression of HLA-E on CD8+, CD4+ and CD14+ cells in conditionallу healthy donors and in the patients with bronchial asthma (BA) and rheumatoid arthritis (RA). Peripheral blood mononuclears (PBMNC) were used as initial biomaterial. PBMNC from RA patients (n = 15), BA (n = 11) and healthy donors were separated from peripheral blood in Ficoll-Urographin density gradient (1.077 g/mL). The cells were then stained with fluorochrome-conjugated monoclonal antibodies: anti-CD3-APC, anti-CD4-APC-Cy7, anti-CD-14-FITC, and anti-HLA-E-PerCP/Cy5. The cell phenotype was analyzed by flow cytometry with FACS Canto II (BD Biosciences, USA). We have found an increased expression of HLA-E on CD8+, CD4+Т cells, like as on CD14+ cells (monocytes) in the RA patients, when compared with BA patients. We have also shown significant differences of HLA-E expression on CD8+Т cells between the conditionally healthy donors and RA patients.

AB - HLA-E is a minor understudied non-classical HLA genes. HLA-E transcription is revealed in many cell types, especially, in immune cells, e.g., T and B cells monocytes, macrophages. In this work, we evaluated expression of HLA-E on CD8+, CD4+ and CD14+ cells in conditionallу healthy donors and in the patients with bronchial asthma (BA) and rheumatoid arthritis (RA). Peripheral blood mononuclears (PBMNC) were used as initial biomaterial. PBMNC from RA patients (n = 15), BA (n = 11) and healthy donors were separated from peripheral blood in Ficoll-Urographin density gradient (1.077 g/mL). The cells were then stained with fluorochrome-conjugated monoclonal antibodies: anti-CD3-APC, anti-CD4-APC-Cy7, anti-CD-14-FITC, and anti-HLA-E-PerCP/Cy5. The cell phenotype was analyzed by flow cytometry with FACS Canto II (BD Biosciences, USA). We have found an increased expression of HLA-E on CD8+, CD4+Т cells, like as on CD14+ cells (monocytes) in the RA patients, when compared with BA patients. We have also shown significant differences of HLA-E expression on CD8+Т cells between the conditionally healthy donors and RA patients.

KW - allergic diseases

KW - autoimmune diseases

KW - bronchial asthma

KW - major histocompatibility complex molecules

KW - non-classical

KW - rheumatoid arthritis

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85174184014&origin=inward&txGid=58089f71e27b18b96f0eb44db02c330b

UR - https://www.mendeley.com/catalogue/706df5a2-93d5-3019-be7f-ee38bf6a5ec7/

U2 - 10.46235/1028-7221-13919-EON

DO - 10.46235/1028-7221-13919-EON

M3 - Article

VL - 26

SP - 527

EP - 532

JO - Russian Journal of Immunology

JF - Russian Journal of Immunology

SN - 2782-7291

IS - 4

ER -

ID: 59180884