TY - JOUR

T1 - Expression of “non-classical” molecules of the main histocompatibility complex in rheumatoid arthritis and bronchial asthma

AU - Boeva, Olga S.

AU - Borisevich, V. I.

AU - Kozlov, V. A.

AU - Demina, D. V.

AU - Sizikov, A. E.

AU - Pashkina, E. A.

N1 - Боева О.С., Борисевич В.И., Козлов В.А., Демина Д.В., Сизиков А.Э., Пашкина Е.А. Экспрессия «неклассических» молекул главного комплекса гистосовместимости при ревматоидном артрите и бронхиальной астме // Российский иммунологический журнал. - 2023. - Т. 26. - №4. - C. 527-532. Публикация для корректировки.

PY - 2023

Y1 - 2023

N2 - HLA-E is a minor understudied non-classical HLA genes. HLA-E transcription is revealed in many cell types, especially, in immune cells, e.g., T and B cells monocytes, macrophages. In this work, we evaluated expression of HLA-E on CD8+, CD4+ and CD14+ cells in conditionallу healthy donors and in the patients with bronchial asthma (BA) and rheumatoid arthritis (RA). Peripheral blood mononuclears (PBMNC) were used as initial biomaterial. PBMNC from RA patients (n = 15), BA (n = 11) and healthy donors were separated from peripheral blood in Ficoll-Urographin density gradient (1.077 g/mL). The cells were then stained with fluorochrome-conjugated monoclonal antibodies: anti-CD3-APC, anti-CD4-APC-Cy7, anti-CD-14-FITC, and anti-HLA-E-PerCP/Cy5. The cell phenotype was analyzed by flow cytometry with FACS Canto II (BD Biosciences, USA). We have found an increased expression of HLA-E on CD8+, CD4+Т cells, like as on CD14+ cells (monocytes) in the RA patients, when compared with BA patients. We have also shown significant differences of HLA-E expression on CD8+Т cells between the conditionally healthy donors and RA patients.

AB - HLA-E is a minor understudied non-classical HLA genes. HLA-E transcription is revealed in many cell types, especially, in immune cells, e.g., T and B cells monocytes, macrophages. In this work, we evaluated expression of HLA-E on CD8+, CD4+ and CD14+ cells in conditionallу healthy donors and in the patients with bronchial asthma (BA) and rheumatoid arthritis (RA). Peripheral blood mononuclears (PBMNC) were used as initial biomaterial. PBMNC from RA patients (n = 15), BA (n = 11) and healthy donors were separated from peripheral blood in Ficoll-Urographin density gradient (1.077 g/mL). The cells were then stained with fluorochrome-conjugated monoclonal antibodies: anti-CD3-APC, anti-CD4-APC-Cy7, anti-CD-14-FITC, and anti-HLA-E-PerCP/Cy5. The cell phenotype was analyzed by flow cytometry with FACS Canto II (BD Biosciences, USA). We have found an increased expression of HLA-E on CD8+, CD4+Т cells, like as on CD14+ cells (monocytes) in the RA patients, when compared with BA patients. We have also shown significant differences of HLA-E expression on CD8+Т cells between the conditionally healthy donors and RA patients.

KW - allergic diseases

KW - autoimmune diseases

KW - bronchial asthma

KW - major histocompatibility complex molecules

KW - non-classical

KW - rheumatoid arthritis

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85174184014&origin=inward&txGid=58089f71e27b18b96f0eb44db02c330b

UR - https://www.mendeley.com/catalogue/706df5a2-93d5-3019-be7f-ee38bf6a5ec7/

U2 - 10.46235/1028-7221-13919-EON

DO - 10.46235/1028-7221-13919-EON

M3 - Article

VL - 26

SP - 527

EP - 532

JO - Russian Journal of Immunology

JF - Russian Journal of Immunology

SN - 2782-7291

IS - 4

ER -