TY - JOUR

T1 - Expression of miR-21 and Its Acat1, Armcx1, and Pten Target Genes in Liver of Female Rats Treated with DDT and Benzo[a]pyrene

AU - Chanyshev, M. D.

AU - Ushakov, D. S.

AU - Gulyaeva, L. F.

PY - 2017/9/14

Y1 - 2017/9/14

N2 - MiR-21 is the most studied cancer-promoting oncomiR, which target numerous tumor suppressor genes associated with proliferation, apoptosis, and invasion. Here we have studied the synthesis of miR-21 and quantified the mRNA and protein levels for miR-21 potential target genes, i.e., Acat1, Armcx1, and Pten, in the livers of female Wistar rats after their treatment with either 1,1-trichloro-2,2-di(4-chlorophenyl)ethane (DDT) or benzo[a]pyrene (BP). The most important finding appears to be the significant decrease in the miR-21 level the day after treatment with DDT with subsequent rebound. These changes are accompanied by an increase and subsequent drop in the levels of mRNAs and proteins of the Acat1, Armcx1, and Pten genes. These observations indicate the involvement of miR-21 in the posttranscriptional regulation of the Acat1, Armcx1, and Pten genes in response to xenobiotics. We hypothesize that the toxic effects of xenobiotics may be indirect and may manifest by inducing epigenetic changes, particularly through the regulation of miRNAs and their target genes.

AB - MiR-21 is the most studied cancer-promoting oncomiR, which target numerous tumor suppressor genes associated with proliferation, apoptosis, and invasion. Here we have studied the synthesis of miR-21 and quantified the mRNA and protein levels for miR-21 potential target genes, i.e., Acat1, Armcx1, and Pten, in the livers of female Wistar rats after their treatment with either 1,1-trichloro-2,2-di(4-chlorophenyl)ethane (DDT) or benzo[a]pyrene (BP). The most important finding appears to be the significant decrease in the miR-21 level the day after treatment with DDT with subsequent rebound. These changes are accompanied by an increase and subsequent drop in the levels of mRNAs and proteins of the Acat1, Armcx1, and Pten genes. These observations indicate the involvement of miR-21 in the posttranscriptional regulation of the Acat1, Armcx1, and Pten genes in response to xenobiotics. We hypothesize that the toxic effects of xenobiotics may be indirect and may manifest by inducing epigenetic changes, particularly through the regulation of miRNAs and their target genes.

KW - Acat1

KW - Armcx1

KW - benzo[a]pyrene

KW - DDT

KW - miR-21

KW - Pten

KW - target genes

KW - Acetyl-CoA C-Acetyltransferase/genetics

KW - Animals

KW - Armadillo Domain Proteins/genetics

KW - Benzo(a)pyrene/toxicity

KW - Carcinogens/toxicity

KW - DDT/toxicity

KW - Epigenesis, Genetic/drug effects

KW - Female

KW - Injections, Intraperitoneal

KW - Liver/drug effects

KW - MicroRNAs/genetics

KW - Oncogene Proteins/genetics

KW - PTEN Phosphohydrolase/genetics

KW - Rats

KW - Rats, Wistar

UR - http://www.scopus.com/inward/record.url?scp=85044239197&partnerID=8YFLogxK

U2 - 10.7868/S0026898417040085

DO - 10.7868/S0026898417040085

M3 - Article

C2 - 28900085

AN - SCOPUS:85044239197

VL - 51

SP - 664

EP - 670

JO - Molekulyarnaya Biologiya

JF - Molekulyarnaya Biologiya

SN - 0026-8984

IS - 4

ER -