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Expression density of receptors to IL-1β in atopic dermatitis. / Alshevskaya, Alina A.; Lopatnikova, Julia A.; Krugleeva, Olga L. и др.
в: Molecular immunology, Том 75, 2016, стр. 92-100.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Expression density of receptors to IL-1β in atopic dermatitis
AU - Alshevskaya, Alina A.
AU - Lopatnikova, Julia A.
AU - Krugleeva, Olga L.
AU - Nepomnyschih, Vera M.
AU - Lukinov, Vitaliy L.
AU - Karaulov, Aleksander V.
AU - Sennikov, Sergey S.
N1 - Publisher Copyright: © 2016 Elsevier Ltd Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - Interleukin 1 (IL-1 β) and the system for regulation of its biological effects play an important role in the development and behavior of inflammatory processes in atopic dermatitis. Notably, cells that are actively involved in the pathological process have altered expression of cytokine receptors. However, standard evaluation of cells by flow cytometry measures only the percentage of cells expressing the appropriate marker, which is not enough for a full assessment of these changes. The aim of this study was to investigate changes in the expression of IL-1β cytokine receptors in patients with atopic dermatitis by both percentage of cells with receptors in various subsets and the absolute number of membrane-bound receptors themselves. It was found that an increase or decrease in the percentage of cells expressing the receptors in subsets of immune cells in patients with atopic dermatitis was not associated with a change in the number of receptors on the cell surface. Moreover, the changes in the percentage of cells and the number of receptors may occur in different directions, as shown for IL-1R2 expression on B cells and IL-1R1 expression for monocytes. Changes in the parameters of IL-1β receptor expressions are associated with disease severity index SCORAD in atopic dermatitis. These findings underline the importance of studying the density of cytokine receptor expression in the pathology.
AB - Interleukin 1 (IL-1 β) and the system for regulation of its biological effects play an important role in the development and behavior of inflammatory processes in atopic dermatitis. Notably, cells that are actively involved in the pathological process have altered expression of cytokine receptors. However, standard evaluation of cells by flow cytometry measures only the percentage of cells expressing the appropriate marker, which is not enough for a full assessment of these changes. The aim of this study was to investigate changes in the expression of IL-1β cytokine receptors in patients with atopic dermatitis by both percentage of cells with receptors in various subsets and the absolute number of membrane-bound receptors themselves. It was found that an increase or decrease in the percentage of cells expressing the receptors in subsets of immune cells in patients with atopic dermatitis was not associated with a change in the number of receptors on the cell surface. Moreover, the changes in the percentage of cells and the number of receptors may occur in different directions, as shown for IL-1R2 expression on B cells and IL-1R1 expression for monocytes. Changes in the parameters of IL-1β receptor expressions are associated with disease severity index SCORAD in atopic dermatitis. These findings underline the importance of studying the density of cytokine receptor expression in the pathology.
KW - Atopic dermatitis
KW - Interleukin-1
KW - Membrane-bound receptors
KW - Receptor expression density
UR - http://www.scopus.com/inward/record.url?scp=84979247407&partnerID=8YFLogxK
U2 - 10.1016/j.molimm.2016.05.015
DO - 10.1016/j.molimm.2016.05.015
M3 - Article
C2 - 27267269
AN - SCOPUS:84979247407
VL - 75
SP - 92
EP - 100
JO - Molecular immunology
JF - Molecular immunology
SN - 0161-5890
ER -
ID: 27071690