Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Exogenous dsRNA-Induced Silencing of the Phytophthora infestans Elicitin Genes inf1 and inf4 Suppresses Its Pathogenicity on Potato Plants. / Ivanov, Artemii A; Golubeva, Tatiana S.
в: Journal of Fungi, Том 9, № 11, 1100, 11.11.2023.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Exogenous dsRNA-Induced Silencing of the Phytophthora infestans Elicitin Genes inf1 and inf4 Suppresses Its Pathogenicity on Potato Plants
AU - Ivanov, Artemii A
AU - Golubeva, Tatiana S
N1 - The research was carried out with the financial support of the Grants of the President of the Russian Federation for state support of young Russian scientists (grant no. MK-4311.2022.5).
PY - 2023/11/11
Y1 - 2023/11/11
N2 - Phytophthora infestans, an Oomycete pathogen, has a devastating impact on potato agriculture, leading to the extensive use of chemical fungicides to prevent its outbreaks. Spraying double-stranded RNAs to suppress specific genes of the pathogen via the RNA interference (RNAi) pathway may provide an environmentally friendly alternative to chemicals. However, this novel approach will require various target genes and application strategies to be tested. Using the L4440 backbone, we have designed two plasmids to express dsRNA targeting inf1 and inf4 genes of P. infestans that are known to contribute to the disease development at different stages. The dsRNA produced by the bacteria was tested on potato explants and demonstrated a statistically significant reduction in lesions five days after inoculation compared to water treatment. The study results allow us to consider our approach to be promising for potato late blight control.
AB - Phytophthora infestans, an Oomycete pathogen, has a devastating impact on potato agriculture, leading to the extensive use of chemical fungicides to prevent its outbreaks. Spraying double-stranded RNAs to suppress specific genes of the pathogen via the RNA interference (RNAi) pathway may provide an environmentally friendly alternative to chemicals. However, this novel approach will require various target genes and application strategies to be tested. Using the L4440 backbone, we have designed two plasmids to express dsRNA targeting inf1 and inf4 genes of P. infestans that are known to contribute to the disease development at different stages. The dsRNA produced by the bacteria was tested on potato explants and demonstrated a statistically significant reduction in lesions five days after inoculation compared to water treatment. The study results allow us to consider our approach to be promising for potato late blight control.
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85178117692&origin=inward&txGid=cab9612de8abcce9bd8a7d3bc717a288
UR - https://www.mendeley.com/catalogue/bec0b3f3-246d-3034-9ddb-242672e375db/
U2 - 10.3390/jof9111100
DO - 10.3390/jof9111100
M3 - Article
C2 - 37998904
VL - 9
JO - Journal of Fungi
JF - Journal of Fungi
SN - 2309-608X
IS - 11
M1 - 1100
ER -
ID: 59230006