TY - JOUR

T1 - Erythrocyte lysis and angle-resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

AU - Yastrebova, Ekaterina S.

AU - Nekrasov, Vyacheslav M.

AU - Gilev, Konstantin V.

AU - Gisich, Alla V.

AU - Abubakirova, Olga A.

AU - Strokotov, Dmitry I.

AU - Chernyshev, Andrey V.

AU - Karpenko, Andrey A.

AU - Maltsev, Valeri P.

N1 - Funding Information: This work was supported by the Russian Science Foundation (grant No. 18-75-10030). Publisher Copyright: © 2022 International Society for Advancement of Cytometry.

PY - 2023/1

Y1 - 2023/1

N2 - Molecular/cell level of gas exchange function assumes the accurate measurement of erythrocyte characteristics and rate constants concerning to molecules involved into the CO2/O2 transport. Unfortunately, common hematology analyzers provide the measurement of eight indices of erythrocytes only and say little about erythrocyte morphology and nothing about rate constants of cellular function. The aim of this study is to demonstrate the ability of the Scanning Flow Cytometer (SFC) in the complete morphological analysis of mature erythrocytes and characterization of erythrocyte function via measurement of lysing kinetics. With this study we are introducing 48 erythrocyte indices. To provide the usability of application of the SFC in clinical diagnosis, we formed four categories of indices which are as follows: content/concentration (9 indices), morphology (26 indices), age (5 indices), and function (8 indices). The erythrocytes of 39 healthy volunteers were analyzed with the SFC to fix the first-ever reference intervals for the new indices introduced. The essential measurable reliability of the presented method is expressed in terms of errors of characteristics of single erythrocytes retrieved from the solution of the inverse light-scattering problem and errors of parameters retrieved from the fitting of the experimental kinetics by molecular-kinetics model of erythrocyte lysis.

AB - Molecular/cell level of gas exchange function assumes the accurate measurement of erythrocyte characteristics and rate constants concerning to molecules involved into the CO2/O2 transport. Unfortunately, common hematology analyzers provide the measurement of eight indices of erythrocytes only and say little about erythrocyte morphology and nothing about rate constants of cellular function. The aim of this study is to demonstrate the ability of the Scanning Flow Cytometer (SFC) in the complete morphological analysis of mature erythrocytes and characterization of erythrocyte function via measurement of lysing kinetics. With this study we are introducing 48 erythrocyte indices. To provide the usability of application of the SFC in clinical diagnosis, we formed four categories of indices which are as follows: content/concentration (9 indices), morphology (26 indices), age (5 indices), and function (8 indices). The erythrocytes of 39 healthy volunteers were analyzed with the SFC to fix the first-ever reference intervals for the new indices introduced. The essential measurable reliability of the presented method is expressed in terms of errors of characteristics of single erythrocytes retrieved from the solution of the inverse light-scattering problem and errors of parameters retrieved from the fitting of the experimental kinetics by molecular-kinetics model of erythrocyte lysis.

KW - erythrocyte indices

KW - gas exchange

KW - inverse problem

KW - light scattering

KW - scanning flow cytometry

KW - Reproducibility of Results

KW - Humans

KW - Erythrocytes

KW - Cell Death

KW - Flow Cytometry/methods

KW - Erythrocyte Indices

UR - http://www.scopus.com/inward/record.url?scp=85127549902&partnerID=8YFLogxK

UR - https://elibrary.ru/item.asp?id=48425258

UR - https://www.mendeley.com/catalogue/896d0d11-0832-3888-afb2-5f54af1e5e76/

U2 - 10.1002/cyto.a.24554

DO - 10.1002/cyto.a.24554

M3 - Article

C2 - 35349217

AN - SCOPUS:85127549902

VL - 103

SP - 39

EP - 53

JO - Cytometry. Part A : the journal of the International Society for Analytical Cytology

JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology

SN - 1552-4922

IS - 1

ER -