TY - JOUR

T1 - Effects of Linoleic Acid on Cryopreservation of IVF-Obtained Domestic Cat Embryos

AU - Okotrub, S. V.

AU - Lebedeva, D. A.

AU - Okotrub, K. A.

AU - Chuyko, E. A.

AU - Brusentsev, E. Yu.

AU - Rakhmanova, T. A.

AU - Amstislavsky, S. Ya.

N1 - The work was carried out with the support of the Russian Foundation of Basic Research, no. 20-34-90093, budget project no. FWNR-2022-0023, using the equipment of the Core Facility Genetic Resources of Laboratory Animals of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, supported by the Ministry of Education and Science of Russia (unique project identifier RFMEFI62119X0023). Part of the work is supported by State Assignment no. 121032400052-6. Effects of Linoleic Acid on Cryopreservation of IVF-Obtained Domestic Cat Embryos / S. V. Okotrub, D. A. Lebedeva, K. A. Okotrub [et al.] // Russian Journal of Developmental Biology. – 2022. – Vol. 53, No. 5. – P. 321-332. – DOI 10.1134/s106236042205006x.

PY - 2022/12/15

Y1 - 2022/12/15

N2 - The role of lipids in mammalian preimplantation development is a fundamental problem of modern embryology. This is especially topical for early development in Carnivora and some other mammals, the species whose oocytes and early embryos are lipid-rich. One of the promising approaches to improve cryotolerance in lipid-rich embryos is to modify their lipidome in vitro. This work aimed at studying the effects of linoleic acid (LA) on the domestic cat embryos during in vitro development and to evaluate how the change in lipid content affects the cryopreservation results. In-vitro-derived embryos were cultured with 400 µM LA for 66 h and randomly distributed between three groups: (1) for the evaluation the lipid amount in embryos and the stage of development, (2) for the estimation of the unsaturation degree in the intracellular lipids and the starting temperature of the lipid phase transition (Т*), and (3) for the cryopreservation by programmed freezing. The rate of in vitro development was not affected in the LA-exposed noncryopreseved embryos. Nile Red staining did not reveal changes in the lipid amount in the LA-treated embryos. However, Raman measurements showed that lipid unsaturation degree was higher in embryos after in vitro culture with LA. In the LA-treated embryos, T* was lower, and the rate of blastocyst development was higher after cryopreservation as compared with controls; this indicates a higher rate of development of such embryos with a modified lipid composition. Thus, the exposure to LA in vitro led to the increase of lipid unsaturation degree and decrease of the T* in cat embryos, which affected their development after cryopreservation.

AB - The role of lipids in mammalian preimplantation development is a fundamental problem of modern embryology. This is especially topical for early development in Carnivora and some other mammals, the species whose oocytes and early embryos are lipid-rich. One of the promising approaches to improve cryotolerance in lipid-rich embryos is to modify their lipidome in vitro. This work aimed at studying the effects of linoleic acid (LA) on the domestic cat embryos during in vitro development and to evaluate how the change in lipid content affects the cryopreservation results. In-vitro-derived embryos were cultured with 400 µM LA for 66 h and randomly distributed between three groups: (1) for the evaluation the lipid amount in embryos and the stage of development, (2) for the estimation of the unsaturation degree in the intracellular lipids and the starting temperature of the lipid phase transition (Т*), and (3) for the cryopreservation by programmed freezing. The rate of in vitro development was not affected in the LA-exposed noncryopreseved embryos. Nile Red staining did not reveal changes in the lipid amount in the LA-treated embryos. However, Raman measurements showed that lipid unsaturation degree was higher in embryos after in vitro culture with LA. In the LA-treated embryos, T* was lower, and the rate of blastocyst development was higher after cryopreservation as compared with controls; this indicates a higher rate of development of such embryos with a modified lipid composition. Thus, the exposure to LA in vitro led to the increase of lipid unsaturation degree and decrease of the T* in cat embryos, which affected their development after cryopreservation.

UR - https://www.mendeley.com/catalogue/81587055-0c85-3fc6-bdee-247617fa7f2d/

UR - https://www.elibrary.ru/item.asp?id=59510880

U2 - 10.1134/s106236042205006x

DO - 10.1134/s106236042205006x

M3 - Article

VL - 53

SP - 321

EP - 332

JO - Russian Journal of Developmental Biology

JF - Russian Journal of Developmental Biology

SN - 1062-3604

IS - 5

ER -