TY - JOUR

T1 - Effects of Bis(imino)acenaphthene (Bian)-Derived Ligands on the Cytotoxicity, DNA Interactions, and Redox Activity of Palladium(II) Bipyridine Complexes

AU - Komlyagina, Veronika I

AU - Romashev, Nikolai F

AU - Besprozvannykh, Victoria K

AU - Arakelyan, Jemma

AU - Wu, Chengnan

AU - Chubarov, Alexey S

AU - Bakaev, Ivan V

AU - Soh, Yee Kiat

AU - Abramov, Pavel A

AU - Cheung, Kin Leung

AU - Kompan'kov, Nikolai B

AU - Ryadun, Aleksey A

AU - Babak, Maria V

AU - Gushchin, Artem L

N1 - Funding: Financial support from the Russian Science Foundation (Grant 21-13-00092) is acknowledged. The anticancer activity experiments were supported by City University of Hong Kong (Project 9610518).

PY - 2023/7/24

Y1 - 2023/7/24

N2 - A series of heteroleptic bipyridine Pd(II) complexes based on 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene (dpp-Bian) or 1,2-bis[(2,4,6-trimethylphenyl)imino]acenaphthene (tmp-Bian) were prepared. All complexes were fully characterized by spectrochemical methods, and their crystal structures were confirmed by X-ray diffraction analysis. The 72 h stability of heteroleptic bipyridine Pd(II) complexes with Bian ligands under physiological conditions was investigated using 1H NMR spectroscopy. The anticancer activity of all complexes was assessed in a panel of cancer cell lines in comparison with uncoordinated ligands and clinically used drugs cisplatin and doxorubicin. The ability of the complexes to bind DNA was investigated using several methods, including EtBr replacement assay, density functional theory calculations, circular dichroism spectroscopy, DNA gel electrophoresis, and TUNEL assay. The electrochemical activity of all complexes and the uncoordinated ligands was studied using cyclic voltammetry, and reactive oxygen species production in cancer cells was investigated using confocal microscopy. Heteroleptic bipyridine PdII-Bian complexes were cytotoxic in a low micromolar concentration range and showed some selectivity toward cancer cells in comparison with noncancerous MRC-5 lung fibroblasts.

AB - A series of heteroleptic bipyridine Pd(II) complexes based on 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene (dpp-Bian) or 1,2-bis[(2,4,6-trimethylphenyl)imino]acenaphthene (tmp-Bian) were prepared. All complexes were fully characterized by spectrochemical methods, and their crystal structures were confirmed by X-ray diffraction analysis. The 72 h stability of heteroleptic bipyridine Pd(II) complexes with Bian ligands under physiological conditions was investigated using 1H NMR spectroscopy. The anticancer activity of all complexes was assessed in a panel of cancer cell lines in comparison with uncoordinated ligands and clinically used drugs cisplatin and doxorubicin. The ability of the complexes to bind DNA was investigated using several methods, including EtBr replacement assay, density functional theory calculations, circular dichroism spectroscopy, DNA gel electrophoresis, and TUNEL assay. The electrochemical activity of all complexes and the uncoordinated ligands was studied using cyclic voltammetry, and reactive oxygen species production in cancer cells was investigated using confocal microscopy. Heteroleptic bipyridine PdII-Bian complexes were cytotoxic in a low micromolar concentration range and showed some selectivity toward cancer cells in comparison with noncancerous MRC-5 lung fibroblasts.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85165546424&origin=inward&txGid=e9d481cf48a48f1cfba50136bdf05b5c

U2 - 10.1021/acs.inorgchem.3c01172

DO - 10.1021/acs.inorgchem.3c01172

M3 - Article

C2 - 37418540

VL - 62

SP - 11541

EP - 11553

JO - Inorganic Chemistry

JF - Inorganic Chemistry

SN - 0020-1669

IS - 29

ER -